Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22404 | 5' | -52 | NC_005056.1 | + | 9439 | 0.68 | 0.613387 |
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Target: 5'- -gGGUcauACCGGCgGCAAuguGACGAUGAg -3' miRNA: 3'- ugCCG---UGGCCGaCGUUu--UUGUUGCUg -5' |
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| 22404 | 5' | -52 | NC_005056.1 | + | 8243 | 0.67 | 0.660585 |
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Target: 5'- cUGuGCGCCGGUUGCAccAGCGccCGGCc -3' miRNA: 3'- uGC-CGUGGCCGACGUuuUUGUu-GCUG- -5' |
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| 22404 | 5' | -52 | NC_005056.1 | + | 7790 | 1.1 | 0.000764 |
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Target: 5'- cACGGCACCGGCUGCAAAAACAACGACg -3' miRNA: 3'- -UGCCGUGGCCGACGUUUUUGUUGCUG- -5' |
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| 22404 | 5' | -52 | NC_005056.1 | + | 7749 | 0.7 | 0.476137 |
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Target: 5'- cCGGUGCCguGGC-GCAGAcgGGCGGCGGCu -3' miRNA: 3'- uGCCGUGG--CCGaCGUUU--UUGUUGCUG- -5' |
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| 22404 | 5' | -52 | NC_005056.1 | + | 7453 | 0.68 | 0.625188 |
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Target: 5'- cCGuaACCGGCacccgGCAGGGACGcccagauauuGCGACa -3' miRNA: 3'- uGCcgUGGCCGa----CGUUUUUGU----------UGCUG- -5' |
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| 22404 | 5' | -52 | NC_005056.1 | + | 4577 | 0.79 | 0.141436 |
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Target: 5'- gGCGGUcgGCUGGCUGCAGAAauaccGCAAUGAa -3' miRNA: 3'- -UGCCG--UGGCCGACGUUUU-----UGUUGCUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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