Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22404 | 5' | -52 | NC_005056.1 | + | 11144 | 0.69 | 0.520544 |
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Target: 5'- cACaGCACCGGCUGgG---GC-GCGACa -3' miRNA: 3'- -UGcCGUGGCCGACgUuuuUGuUGCUG- -5' |
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| 22404 | 5' | -52 | NC_005056.1 | + | 20222 | 0.68 | 0.57814 |
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Target: 5'- uAUGGCACCGGUgGUuauauucCGGCGACc -3' miRNA: 3'- -UGCCGUGGCCGaCGuuuuu--GUUGCUG- -5' |
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| 22404 | 5' | -52 | NC_005056.1 | + | 27632 | 0.68 | 0.601604 |
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Target: 5'- cACuGCAgCGGCUGUuaccGCAugGGCg -3' miRNA: 3'- -UGcCGUgGCCGACGuuuuUGUugCUG- -5' |
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| 22404 | 5' | -52 | NC_005056.1 | + | 17989 | 0.67 | 0.636996 |
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Target: 5'- -aGGCGcuCCGGCgaaGCGGAAACA-CGAa -3' miRNA: 3'- ugCCGU--GGCCGa--CGUUUUUGUuGCUg -5' |
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| 22404 | 5' | -52 | NC_005056.1 | + | 17493 | 0.67 | 0.648799 |
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Target: 5'- aACGGCgucgACCGGCUG---GAGCAACa-- -3' miRNA: 3'- -UGCCG----UGGCCGACguuUUUGUUGcug -5' |
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| 22404 | 5' | -52 | NC_005056.1 | + | 4577 | 0.79 | 0.141436 |
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Target: 5'- gGCGGUcgGCUGGCUGCAGAAauaccGCAAUGAa -3' miRNA: 3'- -UGCCG--UGGCCGACGUUUU-----UGUUGCUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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