Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22553 | 5' | -55.2 | NC_005091.1 | + | 25186 | 0.66 | 0.722668 |
Target: 5'- -aGCCgGCGCGUCGaUGgcGAcCGCCUc -3' miRNA: 3'- aaCGG-CGUGCAGC-GCuuCUuGUGGAc -5' |
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22553 | 5' | -55.2 | NC_005091.1 | + | 53539 | 0.66 | 0.71189 |
Target: 5'- cUUGCCGCACGggCGCGGcuuucuggcgGGAugGUCUa -3' miRNA: 3'- -AACGGCGUGCa-GCGCU----------UCUugUGGAc -5' |
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22553 | 5' | -55.2 | NC_005091.1 | + | 37874 | 0.66 | 0.71189 |
Target: 5'- -gGCUGCGCGcgUGCGGGaAGCGCCa- -3' miRNA: 3'- aaCGGCGUGCa-GCGCUUcUUGUGGac -5' |
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22553 | 5' | -55.2 | NC_005091.1 | + | 46111 | 0.66 | 0.71189 |
Target: 5'- -aGCUGCACGccaaacuccugcUCGUGccGGACGCCg- -3' miRNA: 3'- aaCGGCGUGC------------AGCGCuuCUUGUGGac -5' |
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22553 | 5' | -55.2 | NC_005091.1 | + | 6871 | 0.66 | 0.701031 |
Target: 5'- -aGCUGCGCGUCGgucauCGGAuuuucguccacGAACACCg- -3' miRNA: 3'- aaCGGCGUGCAGC-----GCUU-----------CUUGUGGac -5' |
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22553 | 5' | -55.2 | NC_005091.1 | + | 45712 | 0.66 | 0.701031 |
Target: 5'- -gGCCGCGCGcCGCGccGGcuACugCUc -3' miRNA: 3'- aaCGGCGUGCaGCGCuuCU--UGugGAc -5' |
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22553 | 5' | -55.2 | NC_005091.1 | + | 6950 | 0.66 | 0.690105 |
Target: 5'- -cGCUGCGCGUgCGaCGGAGAcaGCCa- -3' miRNA: 3'- aaCGGCGUGCA-GC-GCUUCUugUGGac -5' |
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22553 | 5' | -55.2 | NC_005091.1 | + | 20384 | 0.67 | 0.668094 |
Target: 5'- -aGUaCGUACGUUGCGAcGAACAUCg- -3' miRNA: 3'- aaCG-GCGUGCAGCGCUuCUUGUGGac -5' |
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22553 | 5' | -55.2 | NC_005091.1 | + | 2019 | 0.67 | 0.668094 |
Target: 5'- -gGCCGCAuCGUCGCGuucGAucuucuCGCCg- -3' miRNA: 3'- aaCGGCGU-GCAGCGCuu-CUu-----GUGGac -5' |
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22553 | 5' | -55.2 | NC_005091.1 | + | 7996 | 0.67 | 0.668094 |
Target: 5'- gUGCCGUACGaacgugugCGCGAGGGGaucgaggucgGCCUGc -3' miRNA: 3'- aACGGCGUGCa-------GCGCUUCUUg---------UGGAC- -5' |
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22553 | 5' | -55.2 | NC_005091.1 | + | 51221 | 0.67 | 0.666989 |
Target: 5'- -cGCCGCGCGUCGCuguGGGAAgcaacgauguaugUugCUGc -3' miRNA: 3'- aaCGGCGUGCAGCGc--UUCUU-------------GugGAC- -5' |
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22553 | 5' | -55.2 | NC_005091.1 | + | 25836 | 0.67 | 0.65703 |
Target: 5'- cUUGCCGUACGaCGCGGcgAGAuCAUCa- -3' miRNA: 3'- -AACGGCGUGCaGCGCU--UCUuGUGGac -5' |
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22553 | 5' | -55.2 | NC_005091.1 | + | 21307 | 0.67 | 0.65703 |
Target: 5'- -cGCCGcCGCGUaCGCGAGGc-CGCCc- -3' miRNA: 3'- aaCGGC-GUGCA-GCGCUUCuuGUGGac -5' |
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22553 | 5' | -55.2 | NC_005091.1 | + | 43185 | 0.67 | 0.645944 |
Target: 5'- --cCCGCuCGUCGCGAA-AGCGCCg- -3' miRNA: 3'- aacGGCGuGCAGCGCUUcUUGUGGac -5' |
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22553 | 5' | -55.2 | NC_005091.1 | + | 30443 | 0.67 | 0.645944 |
Target: 5'- aUGCCgaaGCGCGUUcCGAAGcACACCg- -3' miRNA: 3'- aACGG---CGUGCAGcGCUUCuUGUGGac -5' |
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22553 | 5' | -55.2 | NC_005091.1 | + | 50590 | 0.67 | 0.645944 |
Target: 5'- -aGUCGCgcaacaGCGUCGCGuuauAGAcguCGCCUGu -3' miRNA: 3'- aaCGGCG------UGCAGCGCu---UCUu--GUGGAC- -5' |
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22553 | 5' | -55.2 | NC_005091.1 | + | 26774 | 0.67 | 0.645944 |
Target: 5'- -cGCCGCACcggcUCGCGucuu-CGCCUGa -3' miRNA: 3'- aaCGGCGUGc---AGCGCuucuuGUGGAC- -5' |
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22553 | 5' | -55.2 | NC_005091.1 | + | 38887 | 0.67 | 0.645944 |
Target: 5'- -aGCCG-GCGUCGCGGugAGAagaugcagGCGCUUGa -3' miRNA: 3'- aaCGGCgUGCAGCGCU--UCU--------UGUGGAC- -5' |
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22553 | 5' | -55.2 | NC_005091.1 | + | 25695 | 0.67 | 0.634845 |
Target: 5'- -cGCCGCauucGCGcuuuUCGCGAAGGGCAUg-- -3' miRNA: 3'- aaCGGCG----UGC----AGCGCUUCUUGUGgac -5' |
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22553 | 5' | -55.2 | NC_005091.1 | + | 47050 | 0.67 | 0.634845 |
Target: 5'- aUGCCGCGCucgcgGUCaGCGggGAuCAUCa- -3' miRNA: 3'- aACGGCGUG-----CAG-CGCuuCUuGUGGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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