Results 1 - 20 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22563 | 5' | -58.3 | NC_005091.1 | + | 19797 | 0.67 | 0.459178 |
Target: 5'- --gCGUgaUGCGAUUGCCGACGUgcguuucgaUGCCg -3' miRNA: 3'- aagGCG--ACGUUAACGGCUGCG---------GCGGg -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 26402 | 0.71 | 0.288548 |
Target: 5'- gUCUGCUGCGGcaGCCGcagccuGCGuuGCCUg -3' miRNA: 3'- aAGGCGACGUUaaCGGC------UGCggCGGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 19535 | 0.72 | 0.254499 |
Target: 5'- gUCCgguuGCUGCGcuuucggGCCGAgCGCCGCCg -3' miRNA: 3'- aAGG----CGACGUuaa----CGGCU-GCGGCGGg -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 15762 | 0.72 | 0.248098 |
Target: 5'- -aCCGCagcucgGCAAcacgcaUGCCGACGCC-CCCg -3' miRNA: 3'- aaGGCGa-----CGUUa-----ACGGCUGCGGcGGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 15313 | 0.72 | 0.248098 |
Target: 5'- cUUCCcCUGCGA-UGCCGAccaugugauCGCCuGCCCg -3' miRNA: 3'- -AAGGcGACGUUaACGGCU---------GCGG-CGGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 22844 | 0.72 | 0.226153 |
Target: 5'- -gCCGCUGCucggccgccauauucGAUUGCUGcacgcuCGUCGCCCa -3' miRNA: 3'- aaGGCGACG---------------UUAACGGCu-----GCGGCGGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 39156 | 0.75 | 0.146096 |
Target: 5'- aUUCCGUUGCuugcgGCCGuCGUCGCCUc -3' miRNA: 3'- -AAGGCGACGuuaa-CGGCuGCGGCGGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 22175 | 0.68 | 0.420831 |
Target: 5'- aUCCGCgcaggucgGCAuuUUGCUGGCGgCCGUgCg -3' miRNA: 3'- aAGGCGa-------CGUu-AACGGCUGC-GGCGgG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 44740 | 0.69 | 0.375726 |
Target: 5'- cUUCGUUGC---UGCCuugcCGCCGCCCu -3' miRNA: 3'- aAGGCGACGuuaACGGcu--GCGGCGGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 18965 | 0.68 | 0.439766 |
Target: 5'- gUCCGC----GUUGCCuugaAUGCCGCCCu -3' miRNA: 3'- aAGGCGacguUAACGGc---UGCGGCGGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 44533 | 0.73 | 0.218073 |
Target: 5'- -gUCGCUGCcGUcGCCuucguCGCCGCCCg -3' miRNA: 3'- aaGGCGACGuUAaCGGcu---GCGGCGGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 56291 | 0.67 | 0.479036 |
Target: 5'- -cCCGCUGUAAau-CCGG-GCCGCCa -3' miRNA: 3'- aaGGCGACGUUaacGGCUgCGGCGGg -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 21308 | 0.67 | 0.499306 |
Target: 5'- -gCCGCcGCGuacG-CGAgGCCGCCCg -3' miRNA: 3'- aaGGCGaCGUuaaCgGCUgCGGCGGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 6610 | 0.66 | 0.519949 |
Target: 5'- cUCgCGCgGCGuccgggcUGCCGGCGUucaUGCCCg -3' miRNA: 3'- aAG-GCGaCGUua-----ACGGCUGCG---GCGGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 23973 | 0.66 | 0.519949 |
Target: 5'- -gUgGCUGaaucGUUGCCGACGCgCGUCa -3' miRNA: 3'- aaGgCGACgu--UAACGGCUGCG-GCGGg -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 11425 | 0.66 | 0.530396 |
Target: 5'- gUUUCGCgGCGGccacgucgGCCGACGCuuuCGCCUu -3' miRNA: 3'- -AAGGCGaCGUUaa------CGGCUGCG---GCGGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 4667 | 0.66 | 0.530396 |
Target: 5'- cUCCGC-GCGucguuuuggGCCGGgGCCGUCg -3' miRNA: 3'- aAGGCGaCGUuaa------CGGCUgCGGCGGg -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 11795 | 0.66 | 0.55151 |
Target: 5'- aUCUGUUGguAUcgGCCGcuCGCCGUCg -3' miRNA: 3'- aAGGCGACguUAa-CGGCu-GCGGCGGg -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 25233 | 0.66 | 0.55151 |
Target: 5'- cUUCaCGCcgaGCAcgagGCCGAucaCGUCGCCCa -3' miRNA: 3'- -AAG-GCGa--CGUuaa-CGGCU---GCGGCGGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 51466 | 0.66 | 0.55151 |
Target: 5'- cUUCCGCc-CAcUUGgCGAUGCCGCgCg -3' miRNA: 3'- -AAGGCGacGUuAACgGCUGCGGCGgG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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