Results 21 - 40 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22563 | 5' | -58.3 | NC_005091.1 | + | 26402 | 0.71 | 0.288548 |
Target: 5'- gUCUGCUGCGGcaGCCGcagccuGCGuuGCCUg -3' miRNA: 3'- aAGGCGACGUUaaCGGC------UGCggCGGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 53162 | 0.7 | 0.310643 |
Target: 5'- aUUCCGCUGUAGcgcGCgGGCGCgaGCCUu -3' miRNA: 3'- -AAGGCGACGUUaa-CGgCUGCGg-CGGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 33870 | 0.69 | 0.367105 |
Target: 5'- aUUCGCUa------CCGACGCCGCCCg -3' miRNA: 3'- aAGGCGAcguuaacGGCUGCGGCGGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 14246 | 0.69 | 0.375726 |
Target: 5'- -gCCGCcuUGCG--UGUCGuACGCCGaCCCg -3' miRNA: 3'- aaGGCG--ACGUuaACGGC-UGCGGC-GGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 8333 | 1.1 | 0.000404 |
Target: 5'- aUUCCGCUGCAAUUGCCGACGCCGCCCa -3' miRNA: 3'- -AAGGCGACGUUAACGGCUGCGGCGGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 26683 | 0.74 | 0.171853 |
Target: 5'- -gCUGCUGCGGcgGCCGGCGgCGgCCg -3' miRNA: 3'- aaGGCGACGUUaaCGGCUGCgGCgGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 13961 | 0.7 | 0.303137 |
Target: 5'- -aCgGCUGCAuc-GUCGGCGCCGCa- -3' miRNA: 3'- aaGgCGACGUuaaCGGCUGCGGCGgg -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 52772 | 0.7 | 0.310643 |
Target: 5'- -gCCGUcGCAcugGCCGGCGUgcgCGCCCg -3' miRNA: 3'- aaGGCGaCGUuaaCGGCUGCG---GCGGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 56915 | 0.7 | 0.334001 |
Target: 5'- cUCUGCacgaucgGCAAUUGCCGuCGCUGgCUg -3' miRNA: 3'- aAGGCGa------CGUUAACGGCuGCGGCgGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 25208 | 0.69 | 0.342068 |
Target: 5'- -gCCGCUGCAccGggGUCGAUGCaGCCg -3' miRNA: 3'- aaGGCGACGU--UaaCGGCUGCGgCGGg -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 24682 | 0.69 | 0.350275 |
Target: 5'- cUCCGUcGaCAGggcgcgcgccUUGCaaCGACGCCGCCCc -3' miRNA: 3'- aAGGCGaC-GUU----------AACG--GCUGCGGCGGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 6954 | 0.69 | 0.367105 |
Target: 5'- cUUCCGCUGCGcgUGCgacggaGACaGCCauuucguacgGCCCc -3' miRNA: 3'- -AAGGCGACGUuaACGg-----CUG-CGG----------CGGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 10219 | 0.69 | 0.375726 |
Target: 5'- --gCGCUcgGCGcgUGCCGGCGUaaCGUCCg -3' miRNA: 3'- aagGCGA--CGUuaACGGCUGCG--GCGGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 34860 | 0.68 | 0.393373 |
Target: 5'- -cCCGCUGaagcgGAUcGCCGACGCgCGCggCCg -3' miRNA: 3'- aaGGCGACg----UUAaCGGCUGCG-GCG--GG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 26439 | 0.67 | 0.449414 |
Target: 5'- -aCCGCgcGCGGgccGCCGGgGCUGCCa -3' miRNA: 3'- aaGGCGa-CGUUaa-CGGCUgCGGCGGg -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 10467 | 0.67 | 0.459178 |
Target: 5'- gUCCGCUucgucgccgaagGCGGUauCCG-CGCCGUCCu -3' miRNA: 3'- aAGGCGA------------CGUUAacGGCuGCGGCGGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 52883 | 0.67 | 0.469053 |
Target: 5'- -gCUGCUGCcgcGUgcgGCCuGAuCGCCGCCg -3' miRNA: 3'- aaGGCGACGu--UAa--CGG-CU-GCGGCGGg -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 51021 | 0.67 | 0.479036 |
Target: 5'- -aCCGCgcuUGCGGUcGCUGACGCgGCa- -3' miRNA: 3'- aaGGCG---ACGUUAaCGGCUGCGgCGgg -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 55685 | 0.66 | 0.519949 |
Target: 5'- -gCCGCauCAcUUcGCCGAuCGCCGCCUg -3' miRNA: 3'- aaGGCGacGUuAA-CGGCU-GCGGCGGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 14767 | 0.66 | 0.530396 |
Target: 5'- gUUCCuGUcagUGCGGUUGUCGGC-CCGgCCg -3' miRNA: 3'- -AAGG-CG---ACGUUAACGGCUGcGGCgGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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