Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22563 | 5' | -58.3 | NC_005091.1 | + | 45669 | 0.66 | 0.540918 |
Target: 5'- cUUCGU--CAGgaaGCCGACGCCGgCCa -3' miRNA: 3'- aAGGCGacGUUaa-CGGCUGCGGCgGG- -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 55504 | 0.66 | 0.550448 |
Target: 5'- -gCCGCgacGCuAUUGCgaagcuucgugagCGugGCCGCCa -3' miRNA: 3'- aaGGCGa--CGuUAACG-------------GCugCGGCGGg -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 26305 | 0.66 | 0.562164 |
Target: 5'- -gCCGCU-CAg--GCCGAagcUGCCGCCg -3' miRNA: 3'- aaGGCGAcGUuaaCGGCU---GCGGCGGg -5' |
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22563 | 5' | -58.3 | NC_005091.1 | + | 52206 | 0.66 | 0.562164 |
Target: 5'- gUCCGCaacGCGccccgcGCCGGCgacgGCCGCCUu -3' miRNA: 3'- aAGGCGa--CGUuaa---CGGCUG----CGGCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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