miRNA: 3'- aaGGCGa--CGuUAACG-------------GCugCGGCGGg -5'
miRNA: 3'- aaGGCGacGUuAA-CGGCU-GCGGCGGG- -5'
miRNA: 3'- aaGGCGACGUUaacGGCUgCGGCGGg -5'
miRNA: 3'- aAGGCGa------CGUUAACGGCuGCGGCgGG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22563 | 5' | -58.3 | NC_005091.1 | + | 55504 | 0.66 | 0.550448 |
|
Target: 5'- -gCCGCgacGCuAUUGCgaagcuucgugagCGugGCCGCCa -3' miRNA: 3'- aaGGCGa--CGuUAACG-------------GCugCGGCGGg -5' |
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| 22563 | 5' | -58.3 | NC_005091.1 | + | 55685 | 0.66 | 0.519949 |
|
Target: 5'- -gCCGCauCAcUUcGCCGAuCGCCGCCUg -3' miRNA: 3'- aaGGCGacGUuAA-CGGCU-GCGGCGGG- -5' |
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| 22563 | 5' | -58.3 | NC_005091.1 | + | 56291 | 0.67 | 0.479036 |
|
Target: 5'- -cCCGCUGUAAau-CCGG-GCCGCCa -3' miRNA: 3'- aaGGCGACGUUaacGGCUgCGGCGGg -5' |
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| 22563 | 5' | -58.3 | NC_005091.1 | + | 56915 | 0.7 | 0.334001 |
|
Target: 5'- cUCUGCacgaucgGCAAUUGCCGuCGCUGgCUg -3' miRNA: 3'- aAGGCGa------CGUUAACGGCuGCGGCgGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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