miRNA: 3'- -AAG-GCGa--CGUuaa-CGGCU---GCGGCGGG- -5'
miRNA: 3'- aAGGCGACguUAa-CGGCu-GCGGCGGg -5'
miRNA: 3'- aaGGCGAcGUuaaCGGCU---GCGGCGGg -5'
miRNA: 3'- aAGGCGa--CGUuaa---CGGCUG----CGGCGGG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22563 | 5' | -58.3 | NC_005091.1 | + | 25233 | 0.66 | 0.55151 |
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Target: 5'- cUUCaCGCcgaGCAcgagGCCGAucaCGUCGCCCa -3' miRNA: 3'- -AAG-GCGa--CGUuaa-CGGCU---GCGGCGGG- -5' |
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| 22563 | 5' | -58.3 | NC_005091.1 | + | 11795 | 0.66 | 0.55151 |
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Target: 5'- aUCUGUUGguAUcgGCCGcuCGCCGUCg -3' miRNA: 3'- aAGGCGACguUAa-CGGCu-GCGGCGGg -5' |
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| 22563 | 5' | -58.3 | NC_005091.1 | + | 26305 | 0.66 | 0.562164 |
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Target: 5'- -gCCGCU-CAg--GCCGAagcUGCCGCCg -3' miRNA: 3'- aaGGCGAcGUuaaCGGCU---GCGGCGGg -5' |
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| 22563 | 5' | -58.3 | NC_005091.1 | + | 52206 | 0.66 | 0.562164 |
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Target: 5'- gUCCGCaacGCGccccgcGCCGGCgacgGCCGCCUu -3' miRNA: 3'- aAGGCGa--CGUuaa---CGGCUG----CGGCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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