Results 1 - 20 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22566 | 3' | -56.2 | NC_005091.1 | + | 17000 | 0.66 | 0.765046 |
Target: 5'- cUCGGCGUCuGGUGG-GGC-UGGGCGGn -3' miRNA: 3'- -GGCCGCAGcUCGUCgCUGcACUUGCC- -5' |
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22566 | 3' | -56.2 | NC_005091.1 | + | 16307 | 0.66 | 0.756135 |
Target: 5'- gCCGGCGgccucgucccugaucCGGGC-GCGACG---GCGGg -3' miRNA: 3'- -GGCCGCa--------------GCUCGuCGCUGCacuUGCC- -5' |
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22566 | 3' | -56.2 | NC_005091.1 | + | 20356 | 0.66 | 0.755139 |
Target: 5'- aCGGCGgcuacUCGcaaAGCGGCGACGcGu-CGGc -3' miRNA: 3'- gGCCGC-----AGC---UCGUCGCUGCaCuuGCC- -5' |
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22566 | 3' | -56.2 | NC_005091.1 | + | 20012 | 0.66 | 0.755139 |
Target: 5'- aCGGCGUuaucacugccgUGGGCGGCGAUugguucgaGUGcgccgaGGCGGg -3' miRNA: 3'- gGCCGCA-----------GCUCGUCGCUG--------CAC------UUGCC- -5' |
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22566 | 3' | -56.2 | NC_005091.1 | + | 21586 | 0.66 | 0.734985 |
Target: 5'- gCGGCa--GAGCAcgcuuGCGGCGaUGAACGa -3' miRNA: 3'- gGCCGcagCUCGU-----CGCUGC-ACUUGCc -5' |
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22566 | 3' | -56.2 | NC_005091.1 | + | 32781 | 0.66 | 0.733966 |
Target: 5'- -gGGCGUCGAgaccaccuucguuGCGGCGgaagcgaugGCGUaGGCGGc -3' miRNA: 3'- ggCCGCAGCU-------------CGUCGC---------UGCAcUUGCC- -5' |
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22566 | 3' | -56.2 | NC_005091.1 | + | 31539 | 0.66 | 0.724757 |
Target: 5'- gCUGGCGcUCGuuaCAGCGACGagcguGCGGu -3' miRNA: 3'- -GGCCGC-AGCuc-GUCGCUGCacu--UGCC- -5' |
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22566 | 3' | -56.2 | NC_005091.1 | + | 14597 | 0.66 | 0.724757 |
Target: 5'- cCCGGCaUCGAGCGGC-ACGcuuacCGGc -3' miRNA: 3'- -GGCCGcAGCUCGUCGcUGCacuu-GCC- -5' |
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22566 | 3' | -56.2 | NC_005091.1 | + | 31095 | 0.66 | 0.711334 |
Target: 5'- uCCGGCGcUcaccgugcaacugaCGGGCGGCGcaGCGUGGGaucaGGc -3' miRNA: 3'- -GGCCGC-A--------------GCUCGUCGC--UGCACUUg---CC- -5' |
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22566 | 3' | -56.2 | NC_005091.1 | + | 37081 | 0.67 | 0.704054 |
Target: 5'- uCC-GCGUCGaAGuCAcGgGACGUGAGCGu -3' miRNA: 3'- -GGcCGCAGC-UC-GU-CgCUGCACUUGCc -5' |
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22566 | 3' | -56.2 | NC_005091.1 | + | 43602 | 0.67 | 0.704054 |
Target: 5'- aCGGCaUCcuuuGuCGGaCGGCGUGAGCGGc -3' miRNA: 3'- gGCCGcAGcu--C-GUC-GCUGCACUUGCC- -5' |
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22566 | 3' | -56.2 | NC_005091.1 | + | 55510 | 0.67 | 0.704054 |
Target: 5'- aCGGCGgccgCGAcGCuauuGCGAagcuuCGUGAGCGu -3' miRNA: 3'- gGCCGCa---GCU-CGu---CGCU-----GCACUUGCc -5' |
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22566 | 3' | -56.2 | NC_005091.1 | + | 34962 | 0.67 | 0.700923 |
Target: 5'- gUCGGCuUCGcuuGGCGGCGGCG-GAcuugaaggcgcgucGCGGc -3' miRNA: 3'- -GGCCGcAGC---UCGUCGCUGCaCU--------------UGCC- -5' |
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22566 | 3' | -56.2 | NC_005091.1 | + | 38888 | 0.67 | 0.692549 |
Target: 5'- gCCGGCGUCGcGGUgagaagaugcaGGCGcuugauuACGggcGAACGGu -3' miRNA: 3'- -GGCCGCAGC-UCG-----------UCGC-------UGCa--CUUGCC- -5' |
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22566 | 3' | -56.2 | NC_005091.1 | + | 7900 | 0.67 | 0.683087 |
Target: 5'- aUCGGCuuGUCGuAGCcucGCGACGguucGAugGGu -3' miRNA: 3'- -GGCCG--CAGC-UCGu--CGCUGCa---CUugCC- -5' |
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22566 | 3' | -56.2 | NC_005091.1 | + | 20211 | 0.67 | 0.683087 |
Target: 5'- cCCGGacaCGaAGCGgacGUGGCGUGGAUGGg -3' miRNA: 3'- -GGCCgcaGC-UCGU---CGCUGCACUUGCC- -5' |
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22566 | 3' | -56.2 | NC_005091.1 | + | 5323 | 0.67 | 0.672531 |
Target: 5'- aCGGcCGUgCGGcCAGUGAUGUGAucCGGg -3' miRNA: 3'- gGCC-GCA-GCUcGUCGCUGCACUu-GCC- -5' |
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22566 | 3' | -56.2 | NC_005091.1 | + | 6615 | 0.67 | 0.672531 |
Target: 5'- gCGGCGUcCGGGCuGCcGGCGUucaugcccGAGCGc -3' miRNA: 3'- gGCCGCA-GCUCGuCG-CUGCA--------CUUGCc -5' |
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22566 | 3' | -56.2 | NC_005091.1 | + | 26666 | 0.67 | 0.672531 |
Target: 5'- cCCGGCGUUGAGC-GCaucacGCGcGAACa- -3' miRNA: 3'- -GGCCGCAGCUCGuCGc----UGCaCUUGcc -5' |
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22566 | 3' | -56.2 | NC_005091.1 | + | 52453 | 0.67 | 0.672531 |
Target: 5'- -gGGCGcgcCGGGCGGCGACcccaUGAAUGa -3' miRNA: 3'- ggCCGCa--GCUCGUCGCUGc---ACUUGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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