Results 1 - 20 of 99 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22576 | 5' | -53.6 | NC_005091.1 | + | 30516 | 0.66 | 0.817597 |
Target: 5'- cAGCGGCGCGCucuGCGUCAUgaGCa- -3' miRNA: 3'- -UCGCUGUGCGucgUGUAGUGaaCGcg -5' |
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22576 | 5' | -53.6 | NC_005091.1 | + | 17138 | 0.66 | 0.817597 |
Target: 5'- gAGC-ACGCGCAGCGgCAUUACgUGUu- -3' miRNA: 3'- -UCGcUGUGCGUCGU-GUAGUGaACGcg -5' |
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22576 | 5' | -53.6 | NC_005091.1 | + | 883 | 0.66 | 0.817597 |
Target: 5'- cGCGGCAgCGCAGCGCAcgagaucggcauUCAgUUGaaaGg -3' miRNA: 3'- uCGCUGU-GCGUCGUGU------------AGUgAACg--Cg -5' |
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22576 | 5' | -53.6 | NC_005091.1 | + | 37793 | 0.66 | 0.8119 |
Target: 5'- uGGCGcuucccgcacGCGCGCAGCccuugaauccguuuCGUCACggUGCGg -3' miRNA: 3'- -UCGC----------UGUGCGUCGu-------------GUAGUGa-ACGCg -5' |
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22576 | 5' | -53.6 | NC_005091.1 | + | 39880 | 0.66 | 0.808063 |
Target: 5'- uGCGAUagaACGCGGCcgacCAUCcgGCU-GCGCc -3' miRNA: 3'- uCGCUG---UGCGUCGu---GUAG--UGAaCGCG- -5' |
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22576 | 5' | -53.6 | NC_005091.1 | + | 41627 | 0.66 | 0.808063 |
Target: 5'- cAGCGA-ACGaCAGCAUGUaCGCacGCGCc -3' miRNA: 3'- -UCGCUgUGC-GUCGUGUA-GUGaaCGCG- -5' |
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22576 | 5' | -53.6 | NC_005091.1 | + | 52081 | 0.66 | 0.808063 |
Target: 5'- uGCGACGcCGUAGCGCcgaccAUCGCgaGCu- -3' miRNA: 3'- uCGCUGU-GCGUCGUG-----UAGUGaaCGcg -5' |
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22576 | 5' | -53.6 | NC_005091.1 | + | 9201 | 0.66 | 0.808063 |
Target: 5'- uGGaCGACAUacauucuCGGCACAUCGgggUUGCGCu -3' miRNA: 3'- -UC-GCUGUGc------GUCGUGUAGUg--AACGCG- -5' |
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22576 | 5' | -53.6 | NC_005091.1 | + | 30699 | 0.66 | 0.808063 |
Target: 5'- --aGGCACGCAGUGCuUCg---GCGCg -3' miRNA: 3'- ucgCUGUGCGUCGUGuAGugaaCGCG- -5' |
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22576 | 5' | -53.6 | NC_005091.1 | + | 49714 | 0.66 | 0.807099 |
Target: 5'- cGCGuagcucuGCAUGCggGGCACGUCgGCcgUGCGUg -3' miRNA: 3'- uCGC-------UGUGCG--UCGUGUAG-UGa-ACGCG- -5' |
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22576 | 5' | -53.6 | NC_005091.1 | + | 54619 | 0.66 | 0.798339 |
Target: 5'- uGGCGcACAUGUc-CGCGUagGCUUGCGCc -3' miRNA: 3'- -UCGC-UGUGCGucGUGUAg-UGAACGCG- -5' |
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22576 | 5' | -53.6 | NC_005091.1 | + | 24410 | 0.66 | 0.798339 |
Target: 5'- cGGCGGCACGacggucgacggcCAGCcUAUCGCcgcgaucaagGCGCa -3' miRNA: 3'- -UCGCUGUGC------------GUCGuGUAGUGaa--------CGCG- -5' |
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22576 | 5' | -53.6 | NC_005091.1 | + | 51658 | 0.66 | 0.798339 |
Target: 5'- cGCGcccguCACGCGacGCACGaCACaaUGCGCa -3' miRNA: 3'- uCGCu----GUGCGU--CGUGUaGUGa-ACGCG- -5' |
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22576 | 5' | -53.6 | NC_005091.1 | + | 25632 | 0.66 | 0.798339 |
Target: 5'- uGCGGCGgGCAGCcgAUCGCcgagcuggccGCGCa -3' miRNA: 3'- uCGCUGUgCGUCGugUAGUGaa--------CGCG- -5' |
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22576 | 5' | -53.6 | NC_005091.1 | + | 42659 | 0.66 | 0.797357 |
Target: 5'- gAGCG-UGCGCGGCGCGgacaagcgucuucUCGgCgaGCGCg -3' miRNA: 3'- -UCGCuGUGCGUCGUGU-------------AGU-GaaCGCG- -5' |
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22576 | 5' | -53.6 | NC_005091.1 | + | 14596 | 0.66 | 0.788436 |
Target: 5'- -cCGGCAUcgaGCGGCACGcUUACcgGCGCa -3' miRNA: 3'- ucGCUGUG---CGUCGUGU-AGUGaaCGCG- -5' |
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22576 | 5' | -53.6 | NC_005091.1 | + | 46087 | 0.66 | 0.788436 |
Target: 5'- -cCGGCGCGguGCGCGagCGCUUcuucaGCGUc -3' miRNA: 3'- ucGCUGUGCguCGUGUa-GUGAA-----CGCG- -5' |
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22576 | 5' | -53.6 | NC_005091.1 | + | 6449 | 0.66 | 0.788436 |
Target: 5'- cAGCGuGCGCGCuGCGCugaaggaaGCagGCGCg -3' miRNA: 3'- -UCGC-UGUGCGuCGUGuag-----UGaaCGCG- -5' |
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22576 | 5' | -53.6 | NC_005091.1 | + | 44198 | 0.66 | 0.788436 |
Target: 5'- cGCGACAgugccguaUGCGGCGuCuUCGCcUGCGUu -3' miRNA: 3'- uCGCUGU--------GCGUCGU-GuAGUGaACGCG- -5' |
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22576 | 5' | -53.6 | NC_005091.1 | + | 54955 | 0.66 | 0.778365 |
Target: 5'- aAGCGcCGCGCGGCuguccCGUUuucUGCGCc -3' miRNA: 3'- -UCGCuGUGCGUCGu----GUAGugaACGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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