Results 1 - 20 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22583 | 5' | -56.5 | NC_005091.1 | + | 35200 | 0.66 | 0.673242 |
Target: 5'- ---cAAGCU--GCUGACCGGCCgcucGGCCc -3' miRNA: 3'- guccUUCGGuaCGACUGGCUGG----UCGG- -5' |
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22583 | 5' | -56.5 | NC_005091.1 | + | 9801 | 0.66 | 0.673242 |
Target: 5'- uCAGGAAGCguUaccggguccGCUGGgCGGCUgaaGGCCc -3' miRNA: 3'- -GUCCUUCGguA---------CGACUgGCUGG---UCGG- -5' |
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22583 | 5' | -56.5 | NC_005091.1 | + | 52132 | 0.66 | 0.673242 |
Target: 5'- gCGGGgcGCguUGCgGACgauguaaucgaaCGACCAGCg -3' miRNA: 3'- -GUCCuuCGguACGaCUG------------GCUGGUCGg -5' |
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22583 | 5' | -56.5 | NC_005091.1 | + | 9656 | 0.66 | 0.662351 |
Target: 5'- uCAGGAugguagcGCCGUugaugGCUG-CCGACacggCAGCCu -3' miRNA: 3'- -GUCCUu------CGGUA-----CGACuGGCUG----GUCGG- -5' |
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22583 | 5' | -56.5 | NC_005091.1 | + | 3725 | 0.66 | 0.662351 |
Target: 5'- gCAGGAacGGCagcagGUcGGCCGGuuuCCAGCCa -3' miRNA: 3'- -GUCCU--UCGgua--CGaCUGGCU---GGUCGG- -5' |
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22583 | 5' | -56.5 | NC_005091.1 | + | 4425 | 0.66 | 0.651432 |
Target: 5'- gUAGGcAGGUCGaGCgGGCaCGcCCAGCCg -3' miRNA: 3'- -GUCC-UUCGGUaCGaCUG-GCuGGUCGG- -5' |
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22583 | 5' | -56.5 | NC_005091.1 | + | 2482 | 0.66 | 0.651432 |
Target: 5'- aCGGGAAGCUcggaGUGC-GGCacgaGAUCGGCg -3' miRNA: 3'- -GUCCUUCGG----UACGaCUGg---CUGGUCGg -5' |
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22583 | 5' | -56.5 | NC_005091.1 | + | 10745 | 0.66 | 0.651432 |
Target: 5'- gCGGGAggcgaucaagaaGGCCAUGgaGAUCGAgaAGCg -3' miRNA: 3'- -GUCCU------------UCGGUACgaCUGGCUggUCGg -5' |
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22583 | 5' | -56.5 | NC_005091.1 | + | 5060 | 0.66 | 0.629552 |
Target: 5'- -----uGUCAUGC-GGCCGuACCGGCCu -3' miRNA: 3'- guccuuCGGUACGaCUGGC-UGGUCGG- -5' |
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22583 | 5' | -56.5 | NC_005091.1 | + | 34978 | 0.66 | 0.629552 |
Target: 5'- aAGGggGCCAUug-GAaCGACC-GCCg -3' miRNA: 3'- gUCCuuCGGUAcgaCUgGCUGGuCGG- -5' |
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22583 | 5' | -56.5 | NC_005091.1 | + | 9188 | 0.66 | 0.629552 |
Target: 5'- ---uGAGCC-UGCgucGCCGugCAGCCg -3' miRNA: 3'- guccUUCGGuACGac-UGGCugGUCGG- -5' |
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22583 | 5' | -56.5 | NC_005091.1 | + | 45357 | 0.67 | 0.60768 |
Target: 5'- gCAGGAgAGUaacGagaUGGCCGACCAGaCCa -3' miRNA: 3'- -GUCCU-UCGguaCg--ACUGGCUGGUC-GG- -5' |
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22583 | 5' | -56.5 | NC_005091.1 | + | 3612 | 0.67 | 0.606588 |
Target: 5'- aCGGGAaaaucgucaAGCCcgaugGCUGgaaaccgGCCGACCugcuGCCg -3' miRNA: 3'- -GUCCU---------UCGGua---CGAC-------UGGCUGGu---CGG- -5' |
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22583 | 5' | -56.5 | NC_005091.1 | + | 17868 | 0.67 | 0.596771 |
Target: 5'- cCGGGAc-CUGUGCgaGAUCGGCCGGCg -3' miRNA: 3'- -GUCCUucGGUACGa-CUGGCUGGUCGg -5' |
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22583 | 5' | -56.5 | NC_005091.1 | + | 36499 | 0.67 | 0.596771 |
Target: 5'- -uGGAAgcGCCGUGCUGcGgCGGCCGGaUCg -3' miRNA: 3'- guCCUU--CGGUACGAC-UgGCUGGUC-GG- -5' |
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22583 | 5' | -56.5 | NC_005091.1 | + | 8221 | 0.67 | 0.585892 |
Target: 5'- aAGGAAGCggaCGUGCUGAUCGcgguACUGGgCg -3' miRNA: 3'- gUCCUUCG---GUACGACUGGC----UGGUCgG- -5' |
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22583 | 5' | -56.5 | NC_005091.1 | + | 9088 | 0.67 | 0.585892 |
Target: 5'- uGGGucggcGUCGUGCUGAagcgcaaccCCGAUguGCCg -3' miRNA: 3'- gUCCuu---CGGUACGACU---------GGCUGguCGG- -5' |
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22583 | 5' | -56.5 | NC_005091.1 | + | 4260 | 0.67 | 0.578298 |
Target: 5'- -cGGAAGCCAUucagcuccugcaaggGCUGAcgacucaucCCGuggucguguucgcGCCGGCCg -3' miRNA: 3'- guCCUUCGGUA---------------CGACU---------GGC-------------UGGUCGG- -5' |
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22583 | 5' | -56.5 | NC_005091.1 | + | 20231 | 0.67 | 0.57505 |
Target: 5'- cCAGuGAAGCCGcGCagucgagcgUGAUCGAaUCGGCCg -3' miRNA: 3'- -GUC-CUUCGGUaCG---------ACUGGCU-GGUCGG- -5' |
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22583 | 5' | -56.5 | NC_005091.1 | + | 45567 | 0.68 | 0.542836 |
Target: 5'- uCGGGucGCCGaugugaaGCUGGCCGGCguCGGCUu -3' miRNA: 3'- -GUCCuuCGGUa------CGACUGGCUG--GUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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