Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22612 | 5' | -58 | NC_005091.1 | + | 52135 | 0.66 | 0.591577 |
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Target: 5'- gGGCgCGU--UGCGGA-CGAUGUaaucgaacgaccagCGGCCa -3' miRNA: 3'- -UCG-GCAcuACGCCUaGCUACG--------------GCCGG- -5' |
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| 22612 | 5' | -58 | NC_005091.1 | + | 52279 | 0.68 | 0.480678 |
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Target: 5'- aAGCCccuuaGUGugcguUGCGGAUCGAUucGacagCGGCCa -3' miRNA: 3'- -UCGG-----CACu----ACGCCUAGCUA--Cg---GCCGG- -5' |
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| 22612 | 5' | -58 | NC_005091.1 | + | 52964 | 0.66 | 0.60548 |
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Target: 5'- -aCCGUGGcugguUGUaccuGAUCGcgGCCGGCUg -3' miRNA: 3'- ucGGCACU-----ACGc---CUAGCuaCGGCCGG- -5' |
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| 22612 | 5' | -58 | NC_005091.1 | + | 54099 | 0.67 | 0.521157 |
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Target: 5'- cGCCGUcgcGCuGGAaaagCGAgacGCCGGCCg -3' miRNA: 3'- uCGGCAcuaCG-CCUa---GCUa--CGGCCGG- -5' |
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| 22612 | 5' | -58 | NC_005091.1 | + | 56290 | 0.66 | 0.562897 |
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Target: 5'- cGaCCGgcagGCGGAugaucUCGAUGCCGGaCa -3' miRNA: 3'- uC-GGCacuaCGCCU-----AGCUACGGCCgG- -5' |
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| 22612 | 5' | -58 | NC_005091.1 | + | 56689 | 0.7 | 0.386975 |
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Target: 5'- ---gGUGAUGUGGcAUCGGaggccgUGCCGGUCa -3' miRNA: 3'- ucggCACUACGCC-UAGCU------ACGGCCGG- -5' |
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| 22612 | 5' | -58 | NC_005091.1 | + | 56966 | 0.67 | 0.552365 |
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Target: 5'- cGCgGUGAUGCucGAaugCGAcgcagagcggGCCGGCCg -3' miRNA: 3'- uCGgCACUACGc-CUa--GCUa---------CGGCCGG- -5' |
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| 22612 | 5' | -58 | NC_005091.1 | + | 57333 | 0.69 | 0.414739 |
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Target: 5'- cGCCGgguUGCGGGaugaUCGGcuuguccgguuggugGCCGGCCa -3' miRNA: 3'- uCGGCacuACGCCU----AGCUa--------------CGGCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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