Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22612 | 5' | -58 | NC_005091.1 | + | 34860 | 0.66 | 0.573482 |
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Target: 5'- -cCCGcUGAaGCGGAUCGccgacgcGCgCGGCCg -3' miRNA: 3'- ucGGC-ACUaCGCCUAGCua-----CG-GCCGG- -5' |
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| 22612 | 5' | -58 | NC_005091.1 | + | 25329 | 0.66 | 0.594782 |
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Target: 5'- cGCCGUGG-GCGacguGAUCGGccucgUGCuCGGCg -3' miRNA: 3'- uCGGCACUaCGC----CUAGCU-----ACG-GCCGg -5' |
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| 22612 | 5' | -58 | NC_005091.1 | + | 22864 | 0.66 | 0.594782 |
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Target: 5'- cGuCCGUGcu-CGuGAUCGAUGCCGaGCg -3' miRNA: 3'- uC-GGCACuacGC-CUAGCUACGGC-CGg -5' |
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| 22612 | 5' | -58 | NC_005091.1 | + | 5314 | 0.66 | 0.594782 |
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Target: 5'- cGGCCaGUGAUGU-GAUCcggGgCGGCCg -3' miRNA: 3'- -UCGG-CACUACGcCUAGcuaCgGCCGG- -5' |
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| 22612 | 5' | -58 | NC_005091.1 | + | 52964 | 0.66 | 0.60548 |
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Target: 5'- -aCCGUGGcugguUGUaccuGAUCGcgGCCGGCUg -3' miRNA: 3'- ucGGCACU-----ACGc---CUAGCuaCGGCCGG- -5' |
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| 22612 | 5' | -58 | NC_005091.1 | + | 50073 | 0.66 | 0.60548 |
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Target: 5'- cGGCCuguGUGAUaGCcGAcgaaUCGAgcgcaacgcgUGCCGGCCu -3' miRNA: 3'- -UCGG---CACUA-CGcCU----AGCU----------ACGGCCGG- -5' |
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| 22612 | 5' | -58 | NC_005091.1 | + | 13078 | 0.66 | 0.60548 |
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Target: 5'- gAGCCGUGGcugGCGGcaggcaauaCGccGCuCGGCCc -3' miRNA: 3'- -UCGGCACUa--CGCCua-------GCuaCG-GCCGG- -5' |
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| 22612 | 5' | -58 | NC_005091.1 | + | 4225 | 0.66 | 0.616201 |
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Target: 5'- cGGCUGUGGcagaggaugaagUGCGGc---GUGCCgGGCCa -3' miRNA: 3'- -UCGGCACU------------ACGCCuagcUACGG-CCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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