Results 1 - 20 of 52 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22669 | 5' | -58.5 | NC_005091.1 | + | 51195 | 0.66 | 0.60194 |
Target: 5'- cCACgggCGcGaCGGCGACGAUuuagggUCGUACGUGa -3' miRNA: 3'- -GUGa--GC-C-GUCGCUGCUG------GGCAUGCGC- -5' |
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22669 | 5' | -58.5 | NC_005091.1 | + | 16369 | 0.66 | 0.60194 |
Target: 5'- aCACgcgCGGCcaAGCGACGuggaacguGCUCG-GCGCGu -3' miRNA: 3'- -GUGa--GCCG--UCGCUGC--------UGGGCaUGCGC- -5' |
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22669 | 5' | -58.5 | NC_005091.1 | + | 52792 | 0.66 | 0.591361 |
Target: 5'- gACgCGGCGGCGAUcaGG-CCGcACGCGg -3' miRNA: 3'- gUGaGCCGUCGCUG--CUgGGCaUGCGC- -5' |
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22669 | 5' | -58.5 | NC_005091.1 | + | 37029 | 0.66 | 0.591361 |
Target: 5'- aACUCaGCAGCccGCGACuucuCCGUGCGaCGg -3' miRNA: 3'- gUGAGcCGUCGc-UGCUG----GGCAUGC-GC- -5' |
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22669 | 5' | -58.5 | NC_005091.1 | + | 45521 | 0.66 | 0.590304 |
Target: 5'- uCGC-CGGguGUGAUGgggacaauguuguACCCGUuCGCGa -3' miRNA: 3'- -GUGaGCCguCGCUGC-------------UGGGCAuGCGC- -5' |
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22669 | 5' | -58.5 | NC_005091.1 | + | 10219 | 0.66 | 0.579761 |
Target: 5'- gCGCUCGGCGcGUGcCGGCguaacguCCGUcucGCGCGc -3' miRNA: 3'- -GUGAGCCGU-CGCuGCUG-------GGCA---UGCGC- -5' |
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22669 | 5' | -58.5 | NC_005091.1 | + | 13593 | 0.66 | 0.570305 |
Target: 5'- gGCgUCGaGCAGCGACGugaacgguCUCGgcgGCGCa -3' miRNA: 3'- gUG-AGC-CGUCGCUGCu-------GGGCa--UGCGc -5' |
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22669 | 5' | -58.5 | NC_005091.1 | + | 55521 | 0.66 | 0.559845 |
Target: 5'- aCGCcCGGCuGaCGGCGGCCgCG-ACGCu -3' miRNA: 3'- -GUGaGCCGuC-GCUGCUGG-GCaUGCGc -5' |
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22669 | 5' | -58.5 | NC_005091.1 | + | 31864 | 0.66 | 0.559845 |
Target: 5'- gCGCUCGuCGGCGGCcuGCUCGUGcCGUGg -3' miRNA: 3'- -GUGAGCcGUCGCUGc-UGGGCAU-GCGC- -5' |
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22669 | 5' | -58.5 | NC_005091.1 | + | 18137 | 0.66 | 0.559845 |
Target: 5'- aCACgUCGGuCGGCGcgagcuucGCGGCCCGgcgaucgaGCGUGa -3' miRNA: 3'- -GUG-AGCC-GUCGC--------UGCUGGGCa-------UGCGC- -5' |
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22669 | 5' | -58.5 | NC_005091.1 | + | 26221 | 0.67 | 0.539092 |
Target: 5'- aCGCggCGGCAGCuuCGGCCUGa--GCGg -3' miRNA: 3'- -GUGa-GCCGUCGcuGCUGGGCaugCGC- -5' |
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22669 | 5' | -58.5 | NC_005091.1 | + | 18176 | 0.67 | 0.539092 |
Target: 5'- aGCUCGGCAuCGAgaacaUGAUCC-UGCGCGc -3' miRNA: 3'- gUGAGCCGUcGCU-----GCUGGGcAUGCGC- -5' |
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22669 | 5' | -58.5 | NC_005091.1 | + | 6178 | 0.67 | 0.528813 |
Target: 5'- gGCUUGcgGGCGACGAgCCGaagcgGCGCGa -3' miRNA: 3'- gUGAGCcgUCGCUGCUgGGCa----UGCGC- -5' |
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22669 | 5' | -58.5 | NC_005091.1 | + | 1037 | 0.67 | 0.52779 |
Target: 5'- gCGCggguucCGGCA-CGACGGCUuccggguCGUACGCGa -3' miRNA: 3'- -GUGa-----GCCGUcGCUGCUGG-------GCAUGCGC- -5' |
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22669 | 5' | -58.5 | NC_005091.1 | + | 30005 | 0.67 | 0.52779 |
Target: 5'- aGCgagCGGuCAGCGGCGucugcgcACCCGUgACGgGg -3' miRNA: 3'- gUGa--GCC-GUCGCUGC-------UGGGCA-UGCgC- -5' |
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22669 | 5' | -58.5 | NC_005091.1 | + | 15949 | 0.67 | 0.515562 |
Target: 5'- cCACUUGGCGcucgcggcguccacGCGAUGACCUGcuuguCGCu -3' miRNA: 3'- -GUGAGCCGU--------------CGCUGCUGGGCau---GCGc -5' |
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22669 | 5' | -58.5 | NC_005091.1 | + | 4215 | 0.67 | 0.508482 |
Target: 5'- uGCcCGGCGGCGcgcccgucacCGugCCGUACGUc -3' miRNA: 3'- gUGaGCCGUCGCu---------GCugGGCAUGCGc -5' |
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22669 | 5' | -58.5 | NC_005091.1 | + | 4823 | 0.67 | 0.508482 |
Target: 5'- gCGCUCGGC-GCGGCGGCaaCGgucaaGCGg -3' miRNA: 3'- -GUGAGCCGuCGCUGCUGg-GCaug--CGC- -5' |
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22669 | 5' | -58.5 | NC_005091.1 | + | 55592 | 0.67 | 0.49844 |
Target: 5'- gCGCUCGuGCAgGCGGCGAUCgGcgaagugAUGCGg -3' miRNA: 3'- -GUGAGC-CGU-CGCUGCUGGgCa------UGCGC- -5' |
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22669 | 5' | -58.5 | NC_005091.1 | + | 31717 | 0.68 | 0.488487 |
Target: 5'- cCGCcuUCGGCuGCGcCGAaaugCCGUACGCc -3' miRNA: 3'- -GUG--AGCCGuCGCuGCUg---GGCAUGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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