Results 1 - 20 of 52 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22682 | 5' | -59.6 | NC_005091.1 | + | 12156 | 0.66 | 0.547522 |
Target: 5'- aCGaAGCGCG-CGuaCUGCGGCGggaucagcGCCUu -3' miRNA: 3'- -GC-UUGUGCuGCcgGACGCCGCa-------CGGG- -5' |
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22682 | 5' | -59.6 | NC_005091.1 | + | 33174 | 0.66 | 0.547522 |
Target: 5'- uCGAGCGCGuuauCGGCCgccGCGaGC-UGCUUc -3' miRNA: 3'- -GCUUGUGCu---GCCGGa--CGC-CGcACGGG- -5' |
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22682 | 5' | -59.6 | NC_005091.1 | + | 9176 | 0.66 | 0.547522 |
Target: 5'- aCGAACguAUGAUGaGCCUGCGucgcCGUGCagCCg -3' miRNA: 3'- -GCUUG--UGCUGC-CGGACGCc---GCACG--GG- -5' |
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22682 | 5' | -59.6 | NC_005091.1 | + | 620 | 0.66 | 0.541357 |
Target: 5'- aGGAUAUGGCGGuagacguucaggauuCCcgGCcGCGUGCCUg -3' miRNA: 3'- gCUUGUGCUGCC---------------GGa-CGcCGCACGGG- -5' |
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22682 | 5' | -59.6 | NC_005091.1 | + | 58014 | 0.66 | 0.541357 |
Target: 5'- aGGAUAUGGCGGuagacguucaggauuCCcgGCcGCGUGCCUg -3' miRNA: 3'- gCUUGUGCUGCC---------------GGa-CGcCGCACGGG- -5' |
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22682 | 5' | -59.6 | NC_005091.1 | + | 4233 | 0.66 | 0.534193 |
Target: 5'- -cGACcUGACGGCugUggcagaggaugaagUGCGGCGUGCCg -3' miRNA: 3'- gcUUGuGCUGCCG--G--------------ACGCCGCACGGg -5' |
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22682 | 5' | -59.6 | NC_005091.1 | + | 18994 | 0.66 | 0.527063 |
Target: 5'- uGAAucCGCGACuGGCCgcgaugcucgGCGGaauCGUGCCg -3' miRNA: 3'- gCUU--GUGCUG-CCGGa---------CGCC---GCACGGg -5' |
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22682 | 5' | -59.6 | NC_005091.1 | + | 31626 | 0.66 | 0.51694 |
Target: 5'- uCGAACGuauCGGCg-GCGGCGU-CCCa -3' miRNA: 3'- -GCUUGUgcuGCCGgaCGCCGCAcGGG- -5' |
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22682 | 5' | -59.6 | NC_005091.1 | + | 4305 | 0.66 | 0.496936 |
Target: 5'- uCGuguuCGCGcCGGCCgucgucuuccUGUuucccggcugGGCGUGCCCg -3' miRNA: 3'- -GCuu--GUGCuGCCGG----------ACG----------CCGCACGGG- -5' |
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22682 | 5' | -59.6 | NC_005091.1 | + | 20362 | 0.67 | 0.477288 |
Target: 5'- uCGcGCACGGCGGCUacucgcaaaGCGGCGacgcgucgGCCg -3' miRNA: 3'- -GCuUGUGCUGCCGGa--------CGCCGCa-------CGGg -5' |
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22682 | 5' | -59.6 | NC_005091.1 | + | 9362 | 0.67 | 0.467608 |
Target: 5'- cCGGu--CGGCGGCCaGCGGCGaGCa- -3' miRNA: 3'- -GCUuguGCUGCCGGaCGCCGCaCGgg -5' |
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22682 | 5' | -59.6 | NC_005091.1 | + | 23487 | 0.67 | 0.467608 |
Target: 5'- gCGAugGCGcugcuCGGUCUguucgGCGGCGcugcgucggcUGCCCc -3' miRNA: 3'- -GCUugUGCu----GCCGGA-----CGCCGC----------ACGGG- -5' |
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22682 | 5' | -59.6 | NC_005091.1 | + | 6612 | 0.67 | 0.467608 |
Target: 5'- --cGCGCGGCguccGGgCUGcCGGCGUucauGCCCg -3' miRNA: 3'- gcuUGUGCUG----CCgGAC-GCCGCA----CGGG- -5' |
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22682 | 5' | -59.6 | NC_005091.1 | + | 36302 | 0.67 | 0.467608 |
Target: 5'- aCGcAAUGCG-CGaGCgUcGCGGCGUGCCg -3' miRNA: 3'- -GC-UUGUGCuGC-CGgA-CGCCGCACGGg -5' |
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22682 | 5' | -59.6 | NC_005091.1 | + | 30859 | 0.67 | 0.464724 |
Target: 5'- uCGAcCACGGCGGguuUCUGCgagaacagcgaccaGGCGUcgagGCCCa -3' miRNA: 3'- -GCUuGUGCUGCC---GGACG--------------CCGCA----CGGG- -5' |
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22682 | 5' | -59.6 | NC_005091.1 | + | 1943 | 0.67 | 0.44856 |
Target: 5'- uCGAACGCGACGa--UGCGGCcgccGUGUCg -3' miRNA: 3'- -GCUUGUGCUGCcggACGCCG----CACGGg -5' |
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22682 | 5' | -59.6 | NC_005091.1 | + | 37194 | 0.67 | 0.44856 |
Target: 5'- ----gGCGGCGGCCUGCgauacggucaGGCGU-UCCg -3' miRNA: 3'- gcuugUGCUGCCGGACG----------CCGCAcGGG- -5' |
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22682 | 5' | -59.6 | NC_005091.1 | + | 24411 | 0.67 | 0.439198 |
Target: 5'- uCGGcgGCACGACGGUC-GaCGGCcaGCCUa -3' miRNA: 3'- -GCU--UGUGCUGCCGGaC-GCCGcaCGGG- -5' |
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22682 | 5' | -59.6 | NC_005091.1 | + | 6187 | 0.67 | 0.439198 |
Target: 5'- -uAGCGCGGgGGCUUGCGgGCGacgaGCCg -3' miRNA: 3'- gcUUGUGCUgCCGGACGC-CGCa---CGGg -5' |
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22682 | 5' | -59.6 | NC_005091.1 | + | 27937 | 0.68 | 0.429948 |
Target: 5'- --uGCGCGACGGCCguacGCGuuGUGCg- -3' miRNA: 3'- gcuUGUGCUGCCGGa---CGCcgCACGgg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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