Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22716 | 5' | -54.6 | NC_005091.1 | + | 12448 | 0.66 | 0.769872 |
Target: 5'- gUC-GCACugGcCGCGcUCGUCGCGg- -3' miRNA: 3'- aAGuCGUGugCuGCGC-AGUAGUGCgc -5' |
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22716 | 5' | -54.6 | NC_005091.1 | + | 26134 | 0.66 | 0.769872 |
Target: 5'- aUCAGCgcguGCGCGGCGagcUgAUCGCGCu -3' miRNA: 3'- aAGUCG----UGUGCUGCgc-AgUAGUGCGc -5' |
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22716 | 5' | -54.6 | NC_005091.1 | + | 2422 | 0.66 | 0.769872 |
Target: 5'- aUCuGGCGCACGAC-CGUCuugauggcauUCAgGCGa -3' miRNA: 3'- aAG-UCGUGUGCUGcGCAGu---------AGUgCGC- -5' |
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22716 | 5' | -54.6 | NC_005091.1 | + | 47086 | 0.66 | 0.75962 |
Target: 5'- cUUCAGCgACAUGAUggGCGUCAUC-CuCGg -3' miRNA: 3'- -AAGUCG-UGUGCUG--CGCAGUAGuGcGC- -5' |
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22716 | 5' | -54.6 | NC_005091.1 | + | 51755 | 0.66 | 0.749237 |
Target: 5'- cUUCAGCACuuCG-CGCGaC-UCGCGCa -3' miRNA: 3'- -AAGUCGUGu-GCuGCGCaGuAGUGCGc -5' |
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22716 | 5' | -54.6 | NC_005091.1 | + | 24412 | 0.66 | 0.738733 |
Target: 5'- aUCGGCgGCACGACG-GUCGacggccagccuaUCGcCGCGa -3' miRNA: 3'- aAGUCG-UGUGCUGCgCAGU------------AGU-GCGC- -5' |
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22716 | 5' | -54.6 | NC_005091.1 | + | 41636 | 0.66 | 0.738733 |
Target: 5'- -aCAGCAUguACGcACGCGcCAUCGUGCa -3' miRNA: 3'- aaGUCGUG--UGC-UGCGCaGUAGUGCGc -5' |
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22716 | 5' | -54.6 | NC_005091.1 | + | 29574 | 0.66 | 0.72812 |
Target: 5'- aUCGGCGCGCGccaGCGUCcuacGUCGCuCGc -3' miRNA: 3'- aAGUCGUGUGCug-CGCAG----UAGUGcGC- -5' |
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22716 | 5' | -54.6 | NC_005091.1 | + | 28936 | 0.66 | 0.72812 |
Target: 5'- gUCGGCACGaggccgUGAuCGgGUCGUUGCGCu -3' miRNA: 3'- aAGUCGUGU------GCU-GCgCAGUAGUGCGc -5' |
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22716 | 5' | -54.6 | NC_005091.1 | + | 28137 | 0.67 | 0.71741 |
Target: 5'- uUUCGGCcgGCGugGCGUCcGUCGucuuCGCGu -3' miRNA: 3'- -AAGUCGugUGCugCGCAG-UAGU----GCGC- -5' |
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22716 | 5' | -54.6 | NC_005091.1 | + | 23539 | 0.67 | 0.71741 |
Target: 5'- gUUCcuGCGCcagaugGCGA-GCGUCAUCAUGCa -3' miRNA: 3'- -AAGu-CGUG------UGCUgCGCAGUAGUGCGc -5' |
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22716 | 5' | -54.6 | NC_005091.1 | + | 26658 | 0.67 | 0.706615 |
Target: 5'- gUCGGCgGCcCGGCGUugagcgCAUCACGCGc -3' miRNA: 3'- aAGUCG-UGuGCUGCGca----GUAGUGCGC- -5' |
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22716 | 5' | -54.6 | NC_005091.1 | + | 14100 | 0.67 | 0.706615 |
Target: 5'- -cCAGUACACGGCGgauaUGUgG-CGCGCGa -3' miRNA: 3'- aaGUCGUGUGCUGC----GCAgUaGUGCGC- -5' |
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22716 | 5' | -54.6 | NC_005091.1 | + | 49225 | 0.67 | 0.695746 |
Target: 5'- gUCAGCACGCGAUGgG-CugcUUugGCa -3' miRNA: 3'- aAGUCGUGUGCUGCgCaGu--AGugCGc -5' |
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22716 | 5' | -54.6 | NC_005091.1 | + | 10216 | 0.67 | 0.694655 |
Target: 5'- cUCGGCGCGugcCGGCGUaacguccGUC-UCGCGCGc -3' miRNA: 3'- aAGUCGUGU---GCUGCG-------CAGuAGUGCGC- -5' |
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22716 | 5' | -54.6 | NC_005091.1 | + | 41781 | 0.67 | 0.681524 |
Target: 5'- gUUCgAGCGCgugAUGACGCGUCGUgugcugaagaucaaCACGCc -3' miRNA: 3'- -AAG-UCGUG---UGCUGCGCAGUA--------------GUGCGc -5' |
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22716 | 5' | -54.6 | NC_005091.1 | + | 52786 | 0.67 | 0.662809 |
Target: 5'- aUCAGgGaCGCGGCgGCGaUCAggccgCACGCGg -3' miRNA: 3'- aAGUCgU-GUGCUG-CGC-AGUa----GUGCGC- -5' |
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22716 | 5' | -54.6 | NC_005091.1 | + | 54028 | 0.68 | 0.651757 |
Target: 5'- -cCAGCGCgACGGCGCacGUUuUCACGUa -3' miRNA: 3'- aaGUCGUG-UGCUGCG--CAGuAGUGCGc -5' |
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22716 | 5' | -54.6 | NC_005091.1 | + | 2385 | 0.68 | 0.651757 |
Target: 5'- cUUCGGCGucguCGCGAUGCGUCGUgGCu-- -3' miRNA: 3'- -AAGUCGU----GUGCUGCGCAGUAgUGcgc -5' |
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22716 | 5' | -54.6 | NC_005091.1 | + | 36389 | 0.68 | 0.651757 |
Target: 5'- gUCGGCACgccGCGACGC----UCGCGCa -3' miRNA: 3'- aAGUCGUG---UGCUGCGcaguAGUGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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