Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22731 | 3' | -51.3 | NC_005091.1 | + | 51181 | 0.66 | 0.926511 |
Target: 5'- cCCGGCACGGugCAguuaUGCcggUUUaCCg -3' miRNA: 3'- uGGCUGUGCCugGUaa--ACGa--AAGcGG- -5' |
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22731 | 3' | -51.3 | NC_005091.1 | + | 1001 | 0.66 | 0.926511 |
Target: 5'- uGCuCGACGCGGGCgCGc-UGCUcggCGUCa -3' miRNA: 3'- -UG-GCUGUGCCUG-GUaaACGAaa-GCGG- -5' |
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22731 | 3' | -51.3 | NC_005091.1 | + | 52808 | 0.66 | 0.920509 |
Target: 5'- gGCCGcACGCGGcagcagcaucaaGCCAUU--CaUUCGCCa -3' miRNA: 3'- -UGGC-UGUGCC------------UGGUAAacGaAAGCGG- -5' |
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22731 | 3' | -51.3 | NC_005091.1 | + | 7963 | 0.66 | 0.920509 |
Target: 5'- gACUGACAUGGaagucGCCAUccaagcggGCUcgUGCCg -3' miRNA: 3'- -UGGCUGUGCC-----UGGUAaa------CGAaaGCGG- -5' |
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22731 | 3' | -51.3 | NC_005091.1 | + | 7472 | 0.66 | 0.91422 |
Target: 5'- aGCCGAgGCGcaccaGACCAgucGCUUcguacUUGCCg -3' miRNA: 3'- -UGGCUgUGC-----CUGGUaaaCGAA-----AGCGG- -5' |
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22731 | 3' | -51.3 | NC_005091.1 | + | 6581 | 0.66 | 0.91422 |
Target: 5'- gAUCGAguCGGgcggcACCAUgcggaUGCgUUCGCCa -3' miRNA: 3'- -UGGCUguGCC-----UGGUAa----ACGaAAGCGG- -5' |
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22731 | 3' | -51.3 | NC_005091.1 | + | 44692 | 0.66 | 0.907646 |
Target: 5'- cUUGACGCGGAUCAg--Ga--UCGCCu -3' miRNA: 3'- uGGCUGUGCCUGGUaaaCgaaAGCGG- -5' |
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22731 | 3' | -51.3 | NC_005091.1 | + | 30589 | 0.66 | 0.900788 |
Target: 5'- gUCGGCACGaGACCGUUcuugaaguccaUGaucgCGCCg -3' miRNA: 3'- uGGCUGUGC-CUGGUAA-----------ACgaaaGCGG- -5' |
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22731 | 3' | -51.3 | NC_005091.1 | + | 27997 | 0.66 | 0.900788 |
Target: 5'- gGCCGACACGaccuGAUCGUagGUaucCGCCg -3' miRNA: 3'- -UGGCUGUGC----CUGGUAaaCGaaaGCGG- -5' |
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22731 | 3' | -51.3 | NC_005091.1 | + | 1543 | 0.67 | 0.893649 |
Target: 5'- gGCCGGCcgcCGGAUCGUccgGCUUgucuucgaGCCa -3' miRNA: 3'- -UGGCUGu--GCCUGGUAaa-CGAAag------CGG- -5' |
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22731 | 3' | -51.3 | NC_005091.1 | + | 52089 | 0.67 | 0.893649 |
Target: 5'- cAUUGGCGCGGccguauGCCGUgaaaagGCggccgUCGCCg -3' miRNA: 3'- -UGGCUGUGCC------UGGUAaa----CGaa---AGCGG- -5' |
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22731 | 3' | -51.3 | NC_005091.1 | + | 11432 | 0.67 | 0.893649 |
Target: 5'- gGCgGcCACGucGGCCGac-GCUUUCGCCu -3' miRNA: 3'- -UGgCuGUGC--CUGGUaaaCGAAAGCGG- -5' |
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22731 | 3' | -51.3 | NC_005091.1 | + | 18312 | 0.67 | 0.886236 |
Target: 5'- uCCGACguucugcgcGCGGACC---UGCUUguccUCGUCg -3' miRNA: 3'- uGGCUG---------UGCCUGGuaaACGAA----AGCGG- -5' |
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22731 | 3' | -51.3 | NC_005091.1 | + | 2866 | 0.67 | 0.886236 |
Target: 5'- cGCaCGAUcguguACGGGCCGUUUGCguggugUGCg -3' miRNA: 3'- -UG-GCUG-----UGCCUGGUAAACGaaa---GCGg -5' |
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22731 | 3' | -51.3 | NC_005091.1 | + | 31918 | 0.67 | 0.886236 |
Target: 5'- -aCGACGCGGGCgAacgugGCUgcUGCCa -3' miRNA: 3'- ugGCUGUGCCUGgUaaa--CGAaaGCGG- -5' |
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22731 | 3' | -51.3 | NC_005091.1 | + | 23954 | 0.67 | 0.862397 |
Target: 5'- cGCCGcagauGC-CGGGCCAgugGCUgaaucgUUGCCg -3' miRNA: 3'- -UGGC-----UGuGCCUGGUaaaCGAa-----AGCGG- -5' |
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22731 | 3' | -51.3 | NC_005091.1 | + | 2196 | 0.67 | 0.862397 |
Target: 5'- uACCGGCaccaucaguugcGCGGACUcgUUGagaUCGCUg -3' miRNA: 3'- -UGGCUG------------UGCCUGGuaAACgaaAGCGG- -5' |
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22731 | 3' | -51.3 | NC_005091.1 | + | 10072 | 0.67 | 0.862397 |
Target: 5'- uUCGAgGCGGugCAggUGCUUg-GCUg -3' miRNA: 3'- uGGCUgUGCCugGUaaACGAAagCGG- -5' |
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22731 | 3' | -51.3 | NC_005091.1 | + | 22816 | 0.68 | 0.836311 |
Target: 5'- aACCGGCGCGGcgauauCCGga-GCgaaCGCCg -3' miRNA: 3'- -UGGCUGUGCCu-----GGUaaaCGaaaGCGG- -5' |
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22731 | 3' | -51.3 | NC_005091.1 | + | 48728 | 0.68 | 0.835405 |
Target: 5'- -gCGACACGGagauacgGCCAUgcgUUGCgggcaCGCCa -3' miRNA: 3'- ugGCUGUGCC-------UGGUA---AACGaaa--GCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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