Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22733 | 5' | -49.7 | NC_005091.1 | + | 10291 | 0.66 | 0.961286 |
Target: 5'- uUCACGAaagAGCUggaaaUCgGCGcgAGCgGUCGUa -3' miRNA: 3'- gAGUGCU---UCGA-----AG-CGUuaUCG-CAGCG- -5' |
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22733 | 5' | -49.7 | NC_005091.1 | + | 45863 | 0.66 | 0.957252 |
Target: 5'- -aCACGAAGCggcCGUAcgaGUGGCucuacaagcaGUCGCc -3' miRNA: 3'- gaGUGCUUCGaa-GCGU---UAUCG----------CAGCG- -5' |
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22733 | 5' | -49.7 | NC_005091.1 | + | 39072 | 0.66 | 0.957252 |
Target: 5'- gCUCGcCGggGUgcgCGCGA-GGCGUUGa -3' miRNA: 3'- -GAGU-GCuuCGaa-GCGUUaUCGCAGCg -5' |
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22733 | 5' | -49.7 | NC_005091.1 | + | 11001 | 0.66 | 0.957252 |
Target: 5'- aCUC-UGAugccGGCUUCGCc--GGgGUCGCu -3' miRNA: 3'- -GAGuGCU----UCGAAGCGuuaUCgCAGCG- -5' |
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22733 | 5' | -49.7 | NC_005091.1 | + | 17660 | 0.66 | 0.957252 |
Target: 5'- -gCACGAAGCUgguaacgauUCGaCGGgguuGCGUCGa -3' miRNA: 3'- gaGUGCUUCGA---------AGC-GUUau--CGCAGCg -5' |
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22733 | 5' | -49.7 | NC_005091.1 | + | 45716 | 0.66 | 0.948345 |
Target: 5'- uUCGCGAAGcCUUCGUccgAGaUGUCGa -3' miRNA: 3'- gAGUGCUUC-GAAGCGuuaUC-GCAGCg -5' |
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22733 | 5' | -49.7 | NC_005091.1 | + | 4731 | 0.67 | 0.943461 |
Target: 5'- -gCGCGGAGCUgCGCGAUGcUGUCu- -3' miRNA: 3'- gaGUGCUUCGAaGCGUUAUcGCAGcg -5' |
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22733 | 5' | -49.7 | NC_005091.1 | + | 54944 | 0.67 | 0.941939 |
Target: 5'- cCUUACGggGCaagggUUUGCGgggauuuugggcugAUGGCGcCGCc -3' miRNA: 3'- -GAGUGCuuCG-----AAGCGU--------------UAUCGCaGCG- -5' |
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22733 | 5' | -49.7 | NC_005091.1 | + | 19185 | 0.67 | 0.940391 |
Target: 5'- --uGCGgcGCUUUGCcGUGGCcccgucgaugaacugGUCGCg -3' miRNA: 3'- gagUGCuuCGAAGCGuUAUCG---------------CAGCG- -5' |
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22733 | 5' | -49.7 | NC_005091.1 | + | 20358 | 0.67 | 0.932817 |
Target: 5'- -gCACGGcGGCUacUCGCAAagcGGCGaCGCg -3' miRNA: 3'- gaGUGCU-UCGA--AGCGUUa--UCGCaGCG- -5' |
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22733 | 5' | -49.7 | NC_005091.1 | + | 4998 | 0.67 | 0.932817 |
Target: 5'- -gCGCGAAGCauucgaUCGCGuccuGCGcCGCa -3' miRNA: 3'- gaGUGCUUCGa-----AGCGUuau-CGCaGCG- -5' |
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22733 | 5' | -49.7 | NC_005091.1 | + | 18149 | 0.67 | 0.932817 |
Target: 5'- -gCGCG-AGCUUCGCGGcccGGCGaUCGa -3' miRNA: 3'- gaGUGCuUCGAAGCGUUa--UCGC-AGCg -5' |
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22733 | 5' | -49.7 | NC_005091.1 | + | 23626 | 0.67 | 0.927052 |
Target: 5'- aUCGCGAAuCUUCGCcAUGuccuUGUCGCa -3' miRNA: 3'- gAGUGCUUcGAAGCGuUAUc---GCAGCG- -5' |
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22733 | 5' | -49.7 | NC_005091.1 | + | 28350 | 0.67 | 0.927052 |
Target: 5'- gUCACGcAAGC--CGCcAgcGCGUCGCc -3' miRNA: 3'- gAGUGC-UUCGaaGCGuUauCGCAGCG- -5' |
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22733 | 5' | -49.7 | NC_005091.1 | + | 46545 | 0.67 | 0.927052 |
Target: 5'- gCUCGCGcgacGGCUU-GUcg-AGCGUCGCc -3' miRNA: 3'- -GAGUGCu---UCGAAgCGuuaUCGCAGCG- -5' |
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22733 | 5' | -49.7 | NC_005091.1 | + | 33522 | 0.68 | 0.914635 |
Target: 5'- uUC-CGAaagAGCUUCGCGcAgcGCGcUCGCg -3' miRNA: 3'- gAGuGCU---UCGAAGCGU-UauCGC-AGCG- -5' |
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22733 | 5' | -49.7 | NC_005091.1 | + | 26855 | 0.68 | 0.907985 |
Target: 5'- --gGCGAAGa--CGCGAgccggugcGGCGUCGCa -3' miRNA: 3'- gagUGCUUCgaaGCGUUa-------UCGCAGCG- -5' |
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22733 | 5' | -49.7 | NC_005091.1 | + | 48733 | 0.68 | 0.888583 |
Target: 5'- aUCACGGucauacaGGCUaCGCGGcgggcugcugcguguUGGUGUCGCc -3' miRNA: 3'- gAGUGCU-------UCGAaGCGUU---------------AUCGCAGCG- -5' |
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22733 | 5' | -49.7 | NC_005091.1 | + | 21092 | 0.68 | 0.886299 |
Target: 5'- uUCACGAuac-UCGCAGUcGCGcCGCg -3' miRNA: 3'- gAGUGCUucgaAGCGUUAuCGCaGCG- -5' |
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22733 | 5' | -49.7 | NC_005091.1 | + | 30154 | 0.68 | 0.886299 |
Target: 5'- -gCGCGggGCUUCGCc--GGCuUCGa -3' miRNA: 3'- gaGUGCuuCGAAGCGuuaUCGcAGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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