Results 1 - 20 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22735 | 3' | -57 | NC_005091.1 | + | 27994 | 0.66 | 0.680484 |
Target: 5'- gCCGGCcgacacgaccugAUCGuAGGU-AUCCGCC-GCCa -3' miRNA: 3'- -GGCCG------------UAGC-UCUAgUAGGCGGaCGGc -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 48609 | 0.66 | 0.680484 |
Target: 5'- gCGGcCGUCGGGGUCccAUCCGgCgagGCgCGa -3' miRNA: 3'- gGCC-GUAGCUCUAG--UAGGCgGa--CG-GC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 25720 | 0.66 | 0.680484 |
Target: 5'- gCGGCcagcUCGGcGAUCggCUGCCcGCCGc -3' miRNA: 3'- gGCCGu---AGCU-CUAGuaGGCGGaCGGC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 9348 | 0.66 | 0.669822 |
Target: 5'- gCGGCgagcaGUCGAc--CAUUCGCCgGCCGg -3' miRNA: 3'- gGCCG-----UAGCUcuaGUAGGCGGaCGGC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 14597 | 0.66 | 0.659126 |
Target: 5'- cCCGGCAUCGAGcggCA--CGCUUaCCGg -3' miRNA: 3'- -GGCCGUAGCUCua-GUagGCGGAcGGC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 10114 | 0.66 | 0.659126 |
Target: 5'- cCCGGCGaugaacaaCGuGGUCG-CCGUCgUGCCGg -3' miRNA: 3'- -GGCCGUa-------GCuCUAGUaGGCGG-ACGGC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 26666 | 0.66 | 0.655913 |
Target: 5'- cCCGGCGUUGAGcgCAUCaCGCgcgaacaucugguaCgcgaugGCCGc -3' miRNA: 3'- -GGCCGUAGCUCuaGUAG-GCG--------------Ga-----CGGC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 30298 | 0.66 | 0.648407 |
Target: 5'- aCGuuGUCGAGGUCGaagaCGCCccgGCCGg -3' miRNA: 3'- gGCcgUAGCUCUAGUag--GCGGa--CGGC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 17469 | 0.66 | 0.648407 |
Target: 5'- aCGaGCAcaaUCGAGAUCGUCgCGCggcagaacaUGCCa -3' miRNA: 3'- gGC-CGU---AGCUCUAGUAG-GCGg--------ACGGc -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 23427 | 0.67 | 0.631231 |
Target: 5'- aCCGGCcUCGGGA-CGuggggccuuggcgcuUCCguGCCUGCUGc -3' miRNA: 3'- -GGCCGuAGCUCUaGU---------------AGG--CGGACGGC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 30513 | 0.67 | 0.626935 |
Target: 5'- cCCGcGUAgCGuAGGUCAUCgGCCgGCCc -3' miRNA: 3'- -GGC-CGUaGC-UCUAGUAGgCGGaCGGc -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 28368 | 0.67 | 0.616201 |
Target: 5'- aCCGGCAgcaaggCGaAGGUCAcgcaagCCGCCagcgcgucGCCGg -3' miRNA: 3'- -GGCCGUa-----GC-UCUAGUa-----GGCGGa-------CGGC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 2498 | 0.67 | 0.615128 |
Target: 5'- gCGGCA-CGAGAUCGgcguguuucucaaUCaCGuUCUGCCGc -3' miRNA: 3'- gGCCGUaGCUCUAGU-------------AG-GC-GGACGGC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 41158 | 0.67 | 0.594782 |
Target: 5'- aCGGUAUccuugcCGAGGUCAUcgaaacgauacCCGCgUGCCu -3' miRNA: 3'- gGCCGUA------GCUCUAGUA-----------GGCGgACGGc -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 35807 | 0.67 | 0.594782 |
Target: 5'- aCCGGCAgcuucgCGaAGAagGUCUGCacgGCCGu -3' miRNA: 3'- -GGCCGUa-----GC-UCUagUAGGCGga-CGGC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 18522 | 0.67 | 0.593713 |
Target: 5'- cCCGGCGacaauuccgcauuUCGAcuacaCGUCCgGCCUGCUGa -3' miRNA: 3'- -GGCCGU-------------AGCUcua--GUAGG-CGGACGGC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 1686 | 0.67 | 0.584113 |
Target: 5'- uCCGGCAUCGGc--UAUCgGCCUGaaGg -3' miRNA: 3'- -GGCCGUAGCUcuaGUAGgCGGACggC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 50270 | 0.67 | 0.584113 |
Target: 5'- aCCGGCAUUGcggcGAUCGUCUcaGCCaucGUCGg -3' miRNA: 3'- -GGCCGUAGCu---CUAGUAGG--CGGa--CGGC- -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 33645 | 0.68 | 0.552365 |
Target: 5'- gCGGCAUCGGGAcuUCA-CCaGCCgcgauaGCCu -3' miRNA: 3'- gGCCGUAGCUCU--AGUaGG-CGGa-----CGGc -5' |
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22735 | 3' | -57 | NC_005091.1 | + | 34496 | 0.68 | 0.541894 |
Target: 5'- aUGGUAUCGAc--CGaCCGCCUGUCGa -3' miRNA: 3'- gGCCGUAGCUcuaGUaGGCGGACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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