Results 21 - 32 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22736 | 3' | -54.1 | NC_005091.1 | + | 15850 | 0.69 | 0.5846 |
Target: 5'- gGGCGuCGGCAugcguGUUGCCGAgc-UGCGg -3' miRNA: 3'- gUCGCuGCCGU-----UAACGGCUagcACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 44605 | 0.69 | 0.570288 |
Target: 5'- aGGCGACGGCAGcgacgGCCuguaauaucgucucGA-CGUGCAg -3' miRNA: 3'- gUCGCUGCCGUUaa---CGG--------------CUaGCACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 49881 | 0.7 | 0.551708 |
Target: 5'- gCGGCGAucgcauCGGCAGaaGCCGAUCGaGCc -3' miRNA: 3'- -GUCGCU------GCCGUUaaCGGCUAGCaCGu -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 15999 | 0.7 | 0.563711 |
Target: 5'- -uGCGACGGCuacaucggucagGCCGAcaagcgUCGUGCu -3' miRNA: 3'- guCGCUGCCGuuaa--------CGGCU------AGCACGu -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 52794 | 0.7 | 0.540864 |
Target: 5'- -cGCGGCGGCGAUcagGCCGcacgCG-GCAg -3' miRNA: 3'- guCGCUGCCGUUAa--CGGCua--GCaCGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 54251 | 0.71 | 0.498279 |
Target: 5'- gUAGCacGACgGGCAaaacgcGUUGCCGAUCGcGCGg -3' miRNA: 3'- -GUCG--CUG-CCGU------UAACGGCUAGCaCGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 34258 | 0.72 | 0.447285 |
Target: 5'- -cGCG-CGGC-AUUGCCGAuaUCGUGUc -3' miRNA: 3'- guCGCuGCCGuUAACGGCU--AGCACGu -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 44875 | 0.72 | 0.418116 |
Target: 5'- gGGCGGCGGCAAggcgGCCGAagGcacgaagGCAa -3' miRNA: 3'- gUCGCUGCCGUUaa--CGGCUagCa------CGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 54341 | 0.73 | 0.399325 |
Target: 5'- -cGCGAuCGGCAAcgcguuUUGCCcGUCGUGCu -3' miRNA: 3'- guCGCU-GCCGUU------AACGGcUAGCACGu -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 19795 | 0.73 | 0.381082 |
Target: 5'- gAGCGuGauGCGAUUGCCGA-CGUGCGu -3' miRNA: 3'- gUCGC-UgcCGUUAACGGCUaGCACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 28459 | 0.81 | 0.120443 |
Target: 5'- gAGCGACGGCgAGUUGCCGAaguacaguucgccggUCGUGUu -3' miRNA: 3'- gUCGCUGCCG-UUAACGGCU---------------AGCACGu -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 56829 | 1.09 | 0.001357 |
Target: 5'- cCAGCGACGGCAAUUGCCGAUCGUGCAg -3' miRNA: 3'- -GUCGCUGCCGUUAACGGCUAGCACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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