Results 21 - 32 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22736 | 3' | -54.1 | NC_005091.1 | + | 33329 | 0.67 | 0.738586 |
Target: 5'- gCAGCcgggucuuCGGCAA-UGCCGGUUGcUGCGu -3' miRNA: 3'- -GUCGcu------GCCGUUaACGGCUAGC-ACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 41974 | 0.66 | 0.749138 |
Target: 5'- gUAGCGAagaGGacc-UGCCGA-CGUGCGg -3' miRNA: 3'- -GUCGCUg--CCguuaACGGCUaGCACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 24318 | 0.66 | 0.749138 |
Target: 5'- gCGGCGAUaGGCug--GCCGucgaccGUCGUGCc -3' miRNA: 3'- -GUCGCUG-CCGuuaaCGGC------UAGCACGu -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 37683 | 0.66 | 0.749138 |
Target: 5'- aCAuCGGCGGCAGgucUCGAUgGUGCAu -3' miRNA: 3'- -GUcGCUGCCGUUaacGGCUAgCACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 55611 | 0.66 | 0.75853 |
Target: 5'- uCGGCGaagugauGCGGCAA--GCCGGgaugacgaCGUGCAa -3' miRNA: 3'- -GUCGC-------UGCCGUUaaCGGCUa-------GCACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 56909 | 0.66 | 0.769863 |
Target: 5'- --aCGAuCGGCAAUUGCCG-UCGcuggcUGCGc -3' miRNA: 3'- gucGCU-GCCGUUAACGGCuAGC-----ACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 46540 | 0.66 | 0.769863 |
Target: 5'- -cGCGACGGC--UUGUCGAgCGUcGCc -3' miRNA: 3'- guCGCUGCCGuuAACGGCUaGCA-CGu -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 25623 | 0.66 | 0.769863 |
Target: 5'- aAGCGcgaauGCGGCGGgcaGCCGAUCGccgaGCu -3' miRNA: 3'- gUCGC-----UGCCGUUaa-CGGCUAGCa---CGu -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 10222 | 0.66 | 0.789016 |
Target: 5'- gCGGCGcuCGGCGcgUGCCGGcguaacguccgucUCGcGCGc -3' miRNA: 3'- -GUCGCu-GCCGUuaACGGCU-------------AGCaCGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 13068 | 0.66 | 0.790008 |
Target: 5'- uGGCGGCaGGCAAUacGCCGcUCG-GCc -3' miRNA: 3'- gUCGCUG-CCGUUAa-CGGCuAGCaCGu -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 56950 | 0.66 | 0.790008 |
Target: 5'- -uGCGAC-GCAGagcggGCCGGcCGUGCAc -3' miRNA: 3'- guCGCUGcCGUUaa---CGGCUaGCACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 57380 | 0.66 | 0.793959 |
Target: 5'- aAGCgGGCGGCcaugaaauccucuggAAUUGCgCGGUCaUGCAg -3' miRNA: 3'- gUCG-CUGCCG---------------UUAACG-GCUAGcACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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