Results 21 - 32 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22736 | 3' | -54.1 | NC_005091.1 | + | 15850 | 0.69 | 0.5846 |
Target: 5'- gGGCGuCGGCAugcguGUUGCCGAgc-UGCGg -3' miRNA: 3'- gUCGCuGCCGU-----UAACGGCUagcACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 9351 | 0.69 | 0.591229 |
Target: 5'- cCAGCGGCGaGCAGUcgaccauucgccgGCCGGUCGcccuucGCAg -3' miRNA: 3'- -GUCGCUGC-CGUUAa------------CGGCUAGCa-----CGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 36799 | 0.69 | 0.617854 |
Target: 5'- uGGCGACGGCGA--GCCGuUCGaccucGCGu -3' miRNA: 3'- gUCGCUGCCGUUaaCGGCuAGCa----CGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 26133 | 0.68 | 0.662321 |
Target: 5'- uCAGCGcgugcGCGGCGA--GCUGAUCGcGCu -3' miRNA: 3'- -GUCGC-----UGCCGUUaaCGGCUAGCaCGu -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 18332 | 0.67 | 0.727926 |
Target: 5'- aCGGCGuaccCGGCAAUcccgacUGCCGAccUCGcgaUGCGc -3' miRNA: 3'- -GUCGCu---GCCGUUA------ACGGCU--AGC---ACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 10120 | 0.67 | 0.734335 |
Target: 5'- cCAGCGccCGGCGAUgaacaacguggucGCCG-UCGUGCc -3' miRNA: 3'- -GUCGCu-GCCGUUAa------------CGGCuAGCACGu -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 41974 | 0.66 | 0.749138 |
Target: 5'- gUAGCGAagaGGacc-UGCCGA-CGUGCGg -3' miRNA: 3'- -GUCGCUg--CCguuaACGGCUaGCACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 56909 | 0.66 | 0.769863 |
Target: 5'- --aCGAuCGGCAAUUGCCG-UCGcuggcUGCGc -3' miRNA: 3'- gucGCU-GCCGUUAACGGCuAGC-----ACGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 46540 | 0.66 | 0.769863 |
Target: 5'- -cGCGACGGC--UUGUCGAgCGUcGCc -3' miRNA: 3'- guCGCUGCCGuuAACGGCUaGCA-CGu -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 10222 | 0.66 | 0.789016 |
Target: 5'- gCGGCGcuCGGCGcgUGCCGGcguaacguccgucUCGcGCGc -3' miRNA: 3'- -GUCGCu-GCCGUuaACGGCU-------------AGCaCGU- -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 13068 | 0.66 | 0.790008 |
Target: 5'- uGGCGGCaGGCAAUacGCCGcUCG-GCc -3' miRNA: 3'- gUCGCUG-CCGUUAa-CGGCuAGCaCGu -5' |
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22736 | 3' | -54.1 | NC_005091.1 | + | 56950 | 0.66 | 0.790008 |
Target: 5'- -uGCGAC-GCAGagcggGCCGGcCGUGCAc -3' miRNA: 3'- guCGCUGcCGUUaa---CGGCUaGCACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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