Results 1 - 20 of 99 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22855 | 3' | -53.5 | NC_005137.2 | + | 3 | 0.73 | 0.632496 |
Target: 5'- aGUACGCGGgGCcaGUGAAcAGCgGCGCGu -3' miRNA: 3'- -CGUGCGCCgCG--UAUUUaUCG-CGCGCu -5' |
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22855 | 3' | -53.5 | NC_005137.2 | + | 1541 | 0.66 | 0.956872 |
Target: 5'- uUACGCauGCGCAUuaacguggcaAAAUGGCGCGaUGAu -3' miRNA: 3'- cGUGCGc-CGCGUA----------UUUAUCGCGC-GCU- -5' |
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22855 | 3' | -53.5 | NC_005137.2 | + | 2013 | 0.66 | 0.952873 |
Target: 5'- uGCACgcuGCGGCGCAgacUAuuUAGguCGCGCc- -3' miRNA: 3'- -CGUG---CGCCGCGU---AUuuAUC--GCGCGcu -5' |
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22855 | 3' | -53.5 | NC_005137.2 | + | 7154 | 0.78 | 0.370206 |
Target: 5'- -uGCGCGGCGUGUAAGccGCGCGCu- -3' miRNA: 3'- cgUGCGCCGCGUAUUUauCGCGCGcu -5' |
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22855 | 3' | -53.5 | NC_005137.2 | + | 7177 | 0.71 | 0.765895 |
Target: 5'- cGCGCGCGuugcaGCGCAUuucGAAccGGCGCGUGc -3' miRNA: 3'- -CGUGCGC-----CGCGUA---UUUa-UCGCGCGCu -5' |
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22855 | 3' | -53.5 | NC_005137.2 | + | 10699 | 0.68 | 0.899224 |
Target: 5'- cGUuuCGUGGCGUuc-GAUGGCGUGCa- -3' miRNA: 3'- -CGu-GCGCCGCGuauUUAUCGCGCGcu -5' |
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22855 | 3' | -53.5 | NC_005137.2 | + | 10964 | 0.88 | 0.096523 |
Target: 5'- gGCACuuCGGuCGCGUGAAUAGCGCGCGAc -3' miRNA: 3'- -CGUGc-GCC-GCGUAUUUAUCGCGCGCU- -5' |
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22855 | 3' | -53.5 | NC_005137.2 | + | 11208 | 0.66 | 0.956872 |
Target: 5'- uGCGuuCGCGGuCGCGUAuacAGUuuuuucuGCGCGCa- -3' miRNA: 3'- -CGU--GCGCC-GCGUAU---UUAu------CGCGCGcu -5' |
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22855 | 3' | -53.5 | NC_005137.2 | + | 11296 | 0.66 | 0.939412 |
Target: 5'- cGCuGCGCGGCgggauGCGUAcGUGcuaagcuguguGCGCGCGc -3' miRNA: 3'- -CG-UGCGCCG-----CGUAUuUAU-----------CGCGCGCu -5' |
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22855 | 3' | -53.5 | NC_005137.2 | + | 15818 | 0.67 | 0.911962 |
Target: 5'- cGCACGUGGCaugccuuaaacGCAUcg--GGCGCGUu- -3' miRNA: 3'- -CGUGCGCCG-----------CGUAuuuaUCGCGCGcu -5' |
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22855 | 3' | -53.5 | NC_005137.2 | + | 16912 | 0.66 | 0.939412 |
Target: 5'- cGCACGCGGCGCu-------CG-GCGAa -3' miRNA: 3'- -CGUGCGCCGCGuauuuaucGCgCGCU- -5' |
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22855 | 3' | -53.5 | NC_005137.2 | + | 18767 | 0.68 | 0.897228 |
Target: 5'- aGCACGCGGaacgcCGCGUucaaucaucacuGCGCGCa- -3' miRNA: 3'- -CGUGCGCC-----GCGUAuuuau-------CGCGCGcu -5' |
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22855 | 3' | -53.5 | NC_005137.2 | + | 20200 | 0.68 | 0.899224 |
Target: 5'- gGCGgGCGGCGUcgAuugcGAaAGCGCGUa- -3' miRNA: 3'- -CGUgCGCCGCGuaU----UUaUCGCGCGcu -5' |
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22855 | 3' | -53.5 | NC_005137.2 | + | 20312 | 0.67 | 0.911962 |
Target: 5'- cGCACGC-GCGCAUGacccgauacuuGAUGGgcucCGCGCa- -3' miRNA: 3'- -CGUGCGcCGCGUAU-----------UUAUC----GCGCGcu -5' |
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22855 | 3' | -53.5 | NC_005137.2 | + | 22890 | 0.69 | 0.863166 |
Target: 5'- -gAUGCGGUGCugcGAUcGGCGCGCa- -3' miRNA: 3'- cgUGCGCCGCGuauUUA-UCGCGCGcu -5' |
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22855 | 3' | -53.5 | NC_005137.2 | + | 24305 | 0.66 | 0.948633 |
Target: 5'- gGCGuuUGCGGCuGCGguGGUGGCGgGCa- -3' miRNA: 3'- -CGU--GCGCCG-CGUauUUAUCGCgCGcu -5' |
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22855 | 3' | -53.5 | NC_005137.2 | + | 26512 | 0.69 | 0.830333 |
Target: 5'- -gACGCGGCcgAUGAcuuaaaaguGUGGCGCGCGu -3' miRNA: 3'- cgUGCGCCGcgUAUU---------UAUCGCGCGCu -5' |
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22855 | 3' | -53.5 | NC_005137.2 | + | 26680 | 0.68 | 0.905079 |
Target: 5'- cGCACGUgcgaacguuugauGGCGC-UGAAcGGCG-GCGAa -3' miRNA: 3'- -CGUGCG-------------CCGCGuAUUUaUCGCgCGCU- -5' |
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22855 | 3' | -53.5 | NC_005137.2 | + | 28046 | 0.66 | 0.944147 |
Target: 5'- -aACGUGGCGCGac----GCGCGCu- -3' miRNA: 3'- cgUGCGCCGCGUauuuauCGCGCGcu -5' |
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22855 | 3' | -53.5 | NC_005137.2 | + | 28709 | 0.67 | 0.917957 |
Target: 5'- uCGCGUaauGGUGCAguuUGAAaGGCGCGCa- -3' miRNA: 3'- cGUGCG---CCGCGU---AUUUaUCGCGCGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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