Results 1 - 20 of 61 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22866 | 5' | -56.1 | NC_005137.2 | + | 72804 | 0.66 | 0.885485 |
Target: 5'- gGCGGCGCGCGCuGCcgucuugaUGUuuagcauguaaAUGAGCGc -3' miRNA: 3'- aCGCUGCGCGUG-CGa-------ACA-----------UGCUCGCa -5' |
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22866 | 5' | -56.1 | NC_005137.2 | + | 56497 | 0.66 | 0.885485 |
Target: 5'- cGCGuCGUGU-CGCgUGUACG-GCGUu -3' miRNA: 3'- aCGCuGCGCGuGCGaACAUGCuCGCA- -5' |
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22866 | 5' | -56.1 | NC_005137.2 | + | 16588 | 0.66 | 0.885485 |
Target: 5'- aUG-GAUGUGCGCGCaUUGaUGCGcGGCGg -3' miRNA: 3'- -ACgCUGCGCGUGCG-AAC-AUGC-UCGCa -5' |
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22866 | 5' | -56.1 | NC_005137.2 | + | 71020 | 0.66 | 0.885485 |
Target: 5'- cGCGGCGCcauaguauuuuGCAuaaUGCUagGUACGGGCa- -3' miRNA: 3'- aCGCUGCG-----------CGU---GCGAa-CAUGCUCGca -5' |
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22866 | 5' | -56.1 | NC_005137.2 | + | 115264 | 0.66 | 0.878392 |
Target: 5'- gGCGACGCugGCGcCGCgaccuugGCGAGCa- -3' miRNA: 3'- aCGCUGCG--CGU-GCGaaca---UGCUCGca -5' |
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22866 | 5' | -56.1 | NC_005137.2 | + | 103084 | 0.66 | 0.878392 |
Target: 5'- uUGCGAuuacCGCGUGCGCaaUUGguugcCGGGCGa -3' miRNA: 3'- -ACGCU----GCGCGUGCG--AACau---GCUCGCa -5' |
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22866 | 5' | -56.1 | NC_005137.2 | + | 49214 | 0.66 | 0.878392 |
Target: 5'- cGCGACGCGCgACGCaaaaacucuaGUGCGguucgcAGUGUu -3' miRNA: 3'- aCGCUGCGCG-UGCGaa--------CAUGC------UCGCA- -5' |
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22866 | 5' | -56.1 | NC_005137.2 | + | 26313 | 0.66 | 0.878392 |
Target: 5'- gGCGGCGCGCugGaCUcuaacACGcGCGUc -3' miRNA: 3'- aCGCUGCGCGugC-GAaca--UGCuCGCA- -5' |
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22866 | 5' | -56.1 | NC_005137.2 | + | 49963 | 0.66 | 0.871076 |
Target: 5'- cGCGAUGaGCACuCUUGaUGCGAGUa- -3' miRNA: 3'- aCGCUGCgCGUGcGAAC-AUGCUCGca -5' |
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22866 | 5' | -56.1 | NC_005137.2 | + | 73430 | 0.66 | 0.86354 |
Target: 5'- uUGCGGCGCGCugGgCU----CGAcGCGUu -3' miRNA: 3'- -ACGCUGCGCGugC-GAacauGCU-CGCA- -5' |
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22866 | 5' | -56.1 | NC_005137.2 | + | 31252 | 0.66 | 0.86354 |
Target: 5'- cGCGuCGUGCGCGCUcaacuCGGGCc- -3' miRNA: 3'- aCGCuGCGCGUGCGAacau-GCUCGca -5' |
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22866 | 5' | -56.1 | NC_005137.2 | + | 68800 | 0.66 | 0.861238 |
Target: 5'- cGCGGCaGCGCAaacuCGCUUGUuaaaacuaaagaagACGccAGCGg -3' miRNA: 3'- aCGCUG-CGCGU----GCGAACA--------------UGC--UCGCa -5' |
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22866 | 5' | -56.1 | NC_005137.2 | + | 35529 | 0.66 | 0.855792 |
Target: 5'- gUGCGGCGCGCAUcCgaccgGUACGuuGCa- -3' miRNA: 3'- -ACGCUGCGCGUGcGaa---CAUGCu-CGca -5' |
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22866 | 5' | -56.1 | NC_005137.2 | + | 42671 | 0.66 | 0.855792 |
Target: 5'- cGCGACGCcaaGCGCGCaaucgACGGGUa- -3' miRNA: 3'- aCGCUGCG---CGUGCGaaca-UGCUCGca -5' |
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22866 | 5' | -56.1 | NC_005137.2 | + | 72353 | 0.66 | 0.855005 |
Target: 5'- cUGCGccgcuccGCGUGCACGCUagUGUACacGCGc -3' miRNA: 3'- -ACGC-------UGCGCGUGCGA--ACAUGcuCGCa -5' |
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22866 | 5' | -56.1 | NC_005137.2 | + | 14747 | 0.67 | 0.847836 |
Target: 5'- cGUGACGCaGCACGCgu-UGCaaauAGCGUa -3' miRNA: 3'- aCGCUGCG-CGUGCGaacAUGc---UCGCA- -5' |
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22866 | 5' | -56.1 | NC_005137.2 | + | 67231 | 0.67 | 0.839681 |
Target: 5'- cGCGACcuuCGCACGgUUcUACGGGCa- -3' miRNA: 3'- aCGCUGc--GCGUGCgAAcAUGCUCGca -5' |
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22866 | 5' | -56.1 | NC_005137.2 | + | 1196 | 0.67 | 0.831333 |
Target: 5'- gGUGAagGCGCuguaaACGCUUGaGCGGGUGg -3' miRNA: 3'- aCGCUg-CGCG-----UGCGAACaUGCUCGCa -5' |
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22866 | 5' | -56.1 | NC_005137.2 | + | 1229 | 0.67 | 0.831333 |
Target: 5'- gGUGAagGCGCuacaaACGCUUGaGCGGGUGg -3' miRNA: 3'- aCGCUg-CGCG-----UGCGAACaUGCUCGCa -5' |
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22866 | 5' | -56.1 | NC_005137.2 | + | 1262 | 0.67 | 0.831333 |
Target: 5'- gGUGAagGCGCuacaaACGCUUGaGCGGGUGg -3' miRNA: 3'- aCGCUg-CGCG-----UGCGAACaUGCUCGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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