Results 1 - 20 of 72 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22880 | 5' | -52.6 | NC_005137.2 | + | 70154 | 1.08 | 0.005756 |
Target: 5'- aGCGCUUAACGACGACGCGCCCGUAAAc -3' miRNA: 3'- -CGCGAAUUGCUGCUGCGCGGGCAUUU- -5' |
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22880 | 5' | -52.6 | NC_005137.2 | + | 80246 | 0.8 | 0.319017 |
Target: 5'- uGCGUUUGAUGACGGCGCGaacgCCGUGGc -3' miRNA: 3'- -CGCGAAUUGCUGCUGCGCg---GGCAUUu -5' |
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22880 | 5' | -52.6 | NC_005137.2 | + | 108573 | 0.79 | 0.383435 |
Target: 5'- aCGUgau-CGGCGGCGCGCCCGUGu- -3' miRNA: 3'- cGCGaauuGCUGCUGCGCGGGCAUuu -5' |
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22880 | 5' | -52.6 | NC_005137.2 | + | 30851 | 0.78 | 0.427855 |
Target: 5'- -aGC-UGACGAccCGGCGCGCCCGUAc- -3' miRNA: 3'- cgCGaAUUGCU--GCUGCGCGGGCAUuu -5' |
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22880 | 5' | -52.6 | NC_005137.2 | + | 96405 | 0.78 | 0.437099 |
Target: 5'- cGCGCcgAccgcuacccgcGCGGCGACGCGCCCGc--- -3' miRNA: 3'- -CGCGaaU-----------UGCUGCUGCGCGGGCauuu -5' |
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22880 | 5' | -52.6 | NC_005137.2 | + | 60872 | 0.77 | 0.494829 |
Target: 5'- aGCGCUauuuUAGCGGaauguauuCGACGCGCUCGUGGu -3' miRNA: 3'- -CGCGA----AUUGCU--------GCUGCGCGGGCAUUu -5' |
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22880 | 5' | -52.6 | NC_005137.2 | + | 30738 | 0.76 | 0.52497 |
Target: 5'- gGCGUgaaugccGCGACG-CGCGCCCGUGc- -3' miRNA: 3'- -CGCGaau----UGCUGCuGCGCGGGCAUuu -5' |
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22880 | 5' | -52.6 | NC_005137.2 | + | 62619 | 0.75 | 0.587097 |
Target: 5'- uGUGCU---CGGcCGACGCGUCCGUAGAu -3' miRNA: 3'- -CGCGAauuGCU-GCUGCGCGGGCAUUU- -5' |
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22880 | 5' | -52.6 | NC_005137.2 | + | 89184 | 0.74 | 0.618724 |
Target: 5'- aGCGCUaacaAACG-CGugGCGCgCCGUGAc -3' miRNA: 3'- -CGCGAa---UUGCuGCugCGCG-GGCAUUu -5' |
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22880 | 5' | -52.6 | NC_005137.2 | + | 97990 | 0.74 | 0.629299 |
Target: 5'- gGCGCgcGGCGugGGUGCGCCCGc--- -3' miRNA: 3'- -CGCGaaUUGCugCUGCGCGGGCauuu -5' |
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22880 | 5' | -52.6 | NC_005137.2 | + | 34516 | 0.73 | 0.713215 |
Target: 5'- aGCGCccgugGAC-ACGACGCGCaCCGUGu- -3' miRNA: 3'- -CGCGaa---UUGcUGCUGCGCG-GGCAUuu -5' |
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22880 | 5' | -52.6 | NC_005137.2 | + | 26303 | 0.72 | 0.743736 |
Target: 5'- cGCGUacAACGGCGGCGCGCUgGa--- -3' miRNA: 3'- -CGCGaaUUGCUGCUGCGCGGgCauuu -5' |
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22880 | 5' | -52.6 | NC_005137.2 | + | 107099 | 0.72 | 0.743736 |
Target: 5'- uGCGCUgguuggagGGCGAUGACcCGgCCGUAGAu -3' miRNA: 3'- -CGCGAa-------UUGCUGCUGcGCgGGCAUUU- -5' |
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22880 | 5' | -52.6 | NC_005137.2 | + | 110643 | 0.72 | 0.743736 |
Target: 5'- cGCGCc-GACGGCGuuuggcuUGUGCCCGUGAc -3' miRNA: 3'- -CGCGaaUUGCUGCu------GCGCGGGCAUUu -5' |
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22880 | 5' | -52.6 | NC_005137.2 | + | 130957 | 0.72 | 0.752722 |
Target: 5'- cGCGCUUuuauacaAGCGguuGACGCGCCCGa--- -3' miRNA: 3'- -CGCGAA-------UUGCug-CUGCGCGGGCauuu -5' |
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22880 | 5' | -52.6 | NC_005137.2 | + | 56339 | 0.72 | 0.763579 |
Target: 5'- cGUGCUUGccuguuCGACGACGCGguaaCCCGUc-- -3' miRNA: 3'- -CGCGAAUu-----GCUGCUGCGC----GGGCAuuu -5' |
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22880 | 5' | -52.6 | NC_005137.2 | + | 17518 | 0.71 | 0.782924 |
Target: 5'- uGCGCgUUAACGAgGGCGCGCUUa---- -3' miRNA: 3'- -CGCG-AAUUGCUgCUGCGCGGGcauuu -5' |
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22880 | 5' | -52.6 | NC_005137.2 | + | 32881 | 0.71 | 0.792384 |
Target: 5'- cGCGCgu-ACGACGACGCGguagCCGg--- -3' miRNA: 3'- -CGCGaauUGCUGCUGCGCg---GGCauuu -5' |
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22880 | 5' | -52.6 | NC_005137.2 | + | 20625 | 0.71 | 0.801691 |
Target: 5'- aGCGacaugGGCGugGugGCGCUCGUuGAa -3' miRNA: 3'- -CGCgaa--UUGCugCugCGCGGGCAuUU- -5' |
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22880 | 5' | -52.6 | NC_005137.2 | + | 95290 | 0.71 | 0.819802 |
Target: 5'- cGCGaUUAagcACGACaACGCGCCCGUu-- -3' miRNA: 3'- -CGCgAAU---UGCUGcUGCGCGGGCAuuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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