Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22920 | 3' | -54.3 | NC_005137.2 | + | 78816 | 0.72 | 0.632666 |
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Target: 5'- cGCUAUuaGCCGACACGCAUuugacacAACUCGCa- -3' miRNA: 3'- -UGAUG--CGGCUGUGCGUA-------UUGGGUGga -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 63169 | 0.72 | 0.623236 |
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Target: 5'- aGCU-UGCCGACACGCcgGcGCCgCACCa -3' miRNA: 3'- -UGAuGCGGCUGUGCGuaU-UGG-GUGGa -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 88200 | 0.74 | 0.540317 |
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Target: 5'- gGCcACGCCGuccCACGUgGUGGCCCGCCUc -3' miRNA: 3'- -UGaUGCGGCu--GUGCG-UAUUGGGUGGA- -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 68585 | 0.75 | 0.490357 |
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Target: 5'- gGCUGCGCCGACuCGUucAACCCgugcGCCa -3' miRNA: 3'- -UGAUGCGGCUGuGCGuaUUGGG----UGGa -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 25857 | 0.77 | 0.372143 |
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Target: 5'- gACU-CGCCGACAcCGCGUcccAAUCCACCUu -3' miRNA: 3'- -UGAuGCGGCUGU-GCGUA---UUGGGUGGA- -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 16540 | 1.07 | 0.0046 |
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Target: 5'- gACUACGCCGACACGCAUAACCCACCUc -3' miRNA: 3'- -UGAUGCGGCUGUGCGUAUUGGGUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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