Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 22920 | 3' | -54.3 | NC_005137.2 | + | 59946 | 0.66 | 0.929023 |
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Target: 5'- aGCUggcGCGCCucguuGAUGCGCAU-GCCCguACCUa -3' miRNA: 3'- -UGA---UGCGG-----CUGUGCGUAuUGGG--UGGA- -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 95655 | 0.66 | 0.929023 |
|
Target: 5'- -gUGCGCCGACGgGCAaGugC-GCCUg -3' miRNA: 3'- ugAUGCGGCUGUgCGUaUugGgUGGA- -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 103749 | 0.66 | 0.92355 |
|
Target: 5'- cACUGCGCCGAaucCGUGUuggacACCCAgCg -3' miRNA: 3'- -UGAUGCGGCUgu-GCGUAu----UGGGUgGa -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 50627 | 0.66 | 0.92355 |
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Target: 5'- --cACGCCGGCACGgGgcuGCagCACCUu -3' miRNA: 3'- ugaUGCGGCUGUGCgUau-UGg-GUGGA- -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 5273 | 0.66 | 0.92355 |
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Target: 5'- ---uUGUCGGCGCGCGUcaucaaaagaaAGCUCGCCUc -3' miRNA: 3'- ugauGCGGCUGUGCGUA-----------UUGGGUGGA- -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 106432 | 0.66 | 0.917825 |
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Target: 5'- --gGCGCCGugGgGCAUcGCgUGCCUg -3' miRNA: 3'- ugaUGCGGCugUgCGUAuUGgGUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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