Results 1 - 20 of 54 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22924 | 3' | -55.2 | NC_005137.2 | + | 49207 | 0.66 | 0.929221 |
Target: 5'- uGCGCUCGcAC-GGCuaccuGUCGGUgccgGUCGCu -3' miRNA: 3'- cUGCGAGCaUGuUCG-----CAGCCG----CAGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 130786 | 0.66 | 0.929221 |
Target: 5'- --aGCUCGcuuuUGCuGGCuuuGUCGGCG-CGCg -3' miRNA: 3'- cugCGAGC----AUGuUCG---CAGCCGCaGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 28228 | 0.66 | 0.928696 |
Target: 5'- cGCGCUgcgUGUACAgccgucuGGCGcaucccagCGcGCGUCGCg -3' miRNA: 3'- cUGCGA---GCAUGU-------UCGCa-------GC-CGCAGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 63453 | 0.66 | 0.928696 |
Target: 5'- gGGCGCgCGUugugcccGCGAGCGaCGGCGagacCGUg -3' miRNA: 3'- -CUGCGaGCA-------UGUUCGCaGCCGCa---GCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 110978 | 0.66 | 0.923855 |
Target: 5'- cACGaCUCuUGCGcgucGGCGggCGGCGUCGa -3' miRNA: 3'- cUGC-GAGcAUGU----UCGCa-GCCGCAGCg -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 21136 | 0.66 | 0.918248 |
Target: 5'- -cCGUuugCGUGCAAGCG-CGGCaaaacgCGCg -3' miRNA: 3'- cuGCGa--GCAUGUUCGCaGCCGca----GCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 17312 | 0.66 | 0.918248 |
Target: 5'- uGACGCgcuGUGCGA-CGUgcGCGUCGCa -3' miRNA: 3'- -CUGCGag-CAUGUUcGCAgcCGCAGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 93519 | 0.66 | 0.918248 |
Target: 5'- cGGCGCaaaUGCAcGCGUCGGCcgaaaacugcGUCGUg -3' miRNA: 3'- -CUGCGagcAUGUuCGCAGCCG----------CAGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 96493 | 0.66 | 0.918248 |
Target: 5'- uGCGCgUCGUguccACGGGCGcuaugacuagCGGCG-CGCa -3' miRNA: 3'- cUGCG-AGCA----UGUUCGCa---------GCCGCaGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 1671 | 0.66 | 0.918248 |
Target: 5'- uGGCcgaaUUGaACAAcGCGUCGGCGcCGCg -3' miRNA: 3'- -CUGcg--AGCaUGUU-CGCAGCCGCaGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 2007 | 0.66 | 0.917674 |
Target: 5'- --aGCUCGUGCAcGCuG-CGGCGcagacuauuuaggUCGCg -3' miRNA: 3'- cugCGAGCAUGUuCG-CaGCCGC-------------AGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 46841 | 0.66 | 0.906311 |
Target: 5'- uGCGCgCGUGCGAGCauacUGGCG-CGUg -3' miRNA: 3'- cUGCGaGCAUGUUCGca--GCCGCaGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 41831 | 0.66 | 0.905689 |
Target: 5'- aGGCGUuacgaagUCGU-CGAGC-UCGGCacGUCGCu -3' miRNA: 3'- -CUGCG-------AGCAuGUUCGcAGCCG--CAGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 45277 | 0.67 | 0.893425 |
Target: 5'- gGugGUuaagUUGUGCGAcGCGUCGcGCGccCGCg -3' miRNA: 3'- -CugCG----AGCAUGUU-CGCAGC-CGCa-GCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 98360 | 0.67 | 0.872371 |
Target: 5'- cACGCUUGUGCGccGGU-UUGGCGccgUCGCg -3' miRNA: 3'- cUGCGAGCAUGU--UCGcAGCCGC---AGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 96688 | 0.67 | 0.872371 |
Target: 5'- gGACgGC-CGUACAcaaaaAGCGgguUCGGCGUugaCGCa -3' miRNA: 3'- -CUG-CGaGCAUGU-----UCGC---AGCCGCA---GCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 108272 | 0.67 | 0.864911 |
Target: 5'- gGAUGCg-GUGCu-GCGaUCGGCG-CGCa -3' miRNA: 3'- -CUGCGagCAUGuuCGC-AGCCGCaGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 11195 | 0.67 | 0.864911 |
Target: 5'- aGCGCUUuUGCAAuGCGUUcGCgGUCGCg -3' miRNA: 3'- cUGCGAGcAUGUU-CGCAGcCG-CAGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 112413 | 0.67 | 0.864911 |
Target: 5'- cGGCGCgccaUUGUGCAAGUa-CGGCGUUGg -3' miRNA: 3'- -CUGCG----AGCAUGUUCGcaGCCGCAGCg -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 70156 | 0.68 | 0.857239 |
Target: 5'- gGugGCggugUCGgugaAGGUGUUGGCGUCGg -3' miRNA: 3'- -CugCG----AGCaug-UUCGCAGCCGCAGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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