Results 1 - 20 of 54 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22924 | 3' | -55.2 | NC_005137.2 | + | 1671 | 0.66 | 0.918248 |
Target: 5'- uGGCcgaaUUGaACAAcGCGUCGGCGcCGCg -3' miRNA: 3'- -CUGcg--AGCaUGUU-CGCAGCCGCaGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 2007 | 0.66 | 0.917674 |
Target: 5'- --aGCUCGUGCAcGCuG-CGGCGcagacuauuuaggUCGCg -3' miRNA: 3'- cugCGAGCAUGUuCG-CaGCCGC-------------AGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 2327 | 0.69 | 0.815928 |
Target: 5'- cGCGCUCGUGgAGGCGUgcaUGGcCGUaaacgUGCa -3' miRNA: 3'- cUGCGAGCAUgUUCGCA---GCC-GCA-----GCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 11195 | 0.67 | 0.864911 |
Target: 5'- aGCGCUUuUGCAAuGCGUUcGCgGUCGCg -3' miRNA: 3'- cUGCGAGcAUGUU-CGCAGcCG-CAGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 16353 | 0.71 | 0.701716 |
Target: 5'- uGCGCUCGaACAuaAGCccggUGGCGUCGUc -3' miRNA: 3'- cUGCGAGCaUGU--UCGca--GCCGCAGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 17312 | 0.66 | 0.918248 |
Target: 5'- uGACGCgcuGUGCGA-CGUgcGCGUCGCa -3' miRNA: 3'- -CUGCGag-CAUGUUcGCAgcCGCAGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 17849 | 0.69 | 0.815928 |
Target: 5'- uGGCG-UCGUGuCAAGCGUCGG-GUauUGCg -3' miRNA: 3'- -CUGCgAGCAU-GUUCGCAGCCgCA--GCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 21136 | 0.66 | 0.918248 |
Target: 5'- -cCGUuugCGUGCAAGCG-CGGCaaaacgCGCg -3' miRNA: 3'- cuGCGa--GCAUGUUCGCaGCCGca----GCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 22568 | 0.7 | 0.721779 |
Target: 5'- uGACGCUCc-GC-AGCGUCaGGCG-CGCc -3' miRNA: 3'- -CUGCGAGcaUGuUCGCAG-CCGCaGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 25571 | 0.8 | 0.240075 |
Target: 5'- aGugGCuUCGuUGCAAGCGUUGGCGuUUGCa -3' miRNA: 3'- -CugCG-AGC-AUGUUCGCAGCCGC-AGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 26633 | 0.94 | 0.034027 |
Target: 5'- uGACGCUCGUACAAGCGUgcGCGUCGCa -3' miRNA: 3'- -CUGCGAGCAUGUUCGCAgcCGCAGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 26781 | 0.74 | 0.518588 |
Target: 5'- cACGCUUGUACGAGCGUCauuuuGUGguaCGCg -3' miRNA: 3'- cUGCGAGCAUGUUCGCAGc----CGCa--GCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 28228 | 0.66 | 0.928696 |
Target: 5'- cGCGCUgcgUGUACAgccgucuGGCGcaucccagCGcGCGUCGCg -3' miRNA: 3'- cUGCGA---GCAUGU-------UCGCa-------GC-CGCAGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 31862 | 0.7 | 0.721779 |
Target: 5'- cGACGCgaaCGUGCAcaCGUCGGUGcgacCGCa -3' miRNA: 3'- -CUGCGa--GCAUGUucGCAGCCGCa---GCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 35988 | 0.68 | 0.833014 |
Target: 5'- gGACGCUC-UGCGAGCGuUCcGCGUUa- -3' miRNA: 3'- -CUGCGAGcAUGUUCGC-AGcCGCAGcg -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 38651 | 0.69 | 0.77041 |
Target: 5'- cGACGCUgG-GCAGGCG-CG-CGUUGCg -3' miRNA: 3'- -CUGCGAgCaUGUUCGCaGCcGCAGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 39841 | 0.68 | 0.833014 |
Target: 5'- uGACGUUUGUACAAGaaugcuguucacCGgaaagugCGGCGcCGCu -3' miRNA: 3'- -CUGCGAGCAUGUUC------------GCa------GCCGCaGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 41831 | 0.66 | 0.905689 |
Target: 5'- aGGCGUuacgaagUCGU-CGAGC-UCGGCacGUCGCu -3' miRNA: 3'- -CUGCG-------AGCAuGUUCGcAGCCG--CAGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 43917 | 0.71 | 0.681415 |
Target: 5'- cGCGCUgGUAguAGCGUucguUGGCG-CGCa -3' miRNA: 3'- cUGCGAgCAUguUCGCA----GCCGCaGCG- -5' |
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22924 | 3' | -55.2 | NC_005137.2 | + | 43977 | 0.68 | 0.824559 |
Target: 5'- cGCGUUCG-ACGGGCGcgggCGGCugcucgugccGUCGCu -3' miRNA: 3'- cUGCGAGCaUGUUCGCa---GCCG----------CAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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