Results 1 - 20 of 47 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22966 | 5' | -55.8 | NC_005137.2 | + | 130609 | 0.67 | 0.818582 |
Target: 5'- -uGACGaaaGCgCGGaCACGCGCGCCGAc-- -3' miRNA: 3'- uuCUGC---CG-GUC-GUGCGUGCGGUUuac -5' |
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22966 | 5' | -55.8 | NC_005137.2 | + | 127623 | 0.68 | 0.772166 |
Target: 5'- uGAG-UGGCCGGCACGU-UGCCAGc-- -3' miRNA: 3'- -UUCuGCCGGUCGUGCGuGCGGUUuac -5' |
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22966 | 5' | -55.8 | NC_005137.2 | + | 125050 | 0.68 | 0.732593 |
Target: 5'- gGAGcCGGgCGGCGCG-GCGCCGGAUc -3' miRNA: 3'- -UUCuGCCgGUCGUGCgUGCGGUUUAc -5' |
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22966 | 5' | -55.8 | NC_005137.2 | + | 118649 | 0.66 | 0.852543 |
Target: 5'- uGGACGGUCGgucuacggcGCACaugaGCACGCCAu--- -3' miRNA: 3'- uUCUGCCGGU---------CGUG----CGUGCGGUuuac -5' |
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22966 | 5' | -55.8 | NC_005137.2 | + | 116290 | 1.06 | 0.0034 |
Target: 5'- gAAGACGGCCAGCACGCACGCCAAAUGu -3' miRNA: 3'- -UUCUGCCGGUCGUGCGUGCGGUUUAC- -5' |
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22966 | 5' | -55.8 | NC_005137.2 | + | 114797 | 0.68 | 0.732593 |
Target: 5'- uGGGCGGCUugcGCACGUcgucguCGUCAGAUGu -3' miRNA: 3'- uUCUGCCGGu--CGUGCGu-----GCGGUUUAC- -5' |
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22966 | 5' | -55.8 | NC_005137.2 | + | 113106 | 0.66 | 0.852543 |
Target: 5'- uAGAauaugUGGCaAGCGCGUGCGCCAAc-- -3' miRNA: 3'- uUCU-----GCCGgUCGUGCGUGCGGUUuac -5' |
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22966 | 5' | -55.8 | NC_005137.2 | + | 110626 | 0.72 | 0.494616 |
Target: 5'- -uGACGGCCGuGCACaaaCGCGCCGAcgGc -3' miRNA: 3'- uuCUGCCGGU-CGUGc--GUGCGGUUuaC- -5' |
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22966 | 5' | -55.8 | NC_005137.2 | + | 110456 | 0.7 | 0.622252 |
Target: 5'- --cGCGGCCGGCGCGUAcacggggucacuaacCGCCAGcUGu -3' miRNA: 3'- uucUGCCGGUCGUGCGU---------------GCGGUUuAC- -5' |
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22966 | 5' | -55.8 | NC_005137.2 | + | 108150 | 0.66 | 0.852543 |
Target: 5'- cGGGCGGCggCGGCgucgguauGCGCGCGCCc---- -3' miRNA: 3'- uUCUGCCG--GUCG--------UGCGUGCGGuuuac -5' |
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22966 | 5' | -55.8 | NC_005137.2 | + | 104225 | 0.74 | 0.418796 |
Target: 5'- cGAGcCGGCCAGCACGacgaagGCGCCAc--- -3' miRNA: 3'- -UUCuGCCGGUCGUGCg-----UGCGGUuuac -5' |
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22966 | 5' | -55.8 | NC_005137.2 | + | 103677 | 0.66 | 0.860523 |
Target: 5'- uGAGAUGGCCaAGgGCGaacuuaACGCCAc--- -3' miRNA: 3'- -UUCUGCCGG-UCgUGCg-----UGCGGUuuac -5' |
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22966 | 5' | -55.8 | NC_005137.2 | + | 103060 | 0.67 | 0.80049 |
Target: 5'- -uGACGGCCu---CGUACGCCGuGUGc -3' miRNA: 3'- uuCUGCCGGucguGCGUGCGGUuUAC- -5' |
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22966 | 5' | -55.8 | NC_005137.2 | + | 99966 | 0.66 | 0.835955 |
Target: 5'- --cGCGGCC-GCGCGCcucuucguGCGCCAGc-- -3' miRNA: 3'- uucUGCCGGuCGUGCG--------UGCGGUUuac -5' |
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22966 | 5' | -55.8 | NC_005137.2 | + | 99332 | 0.66 | 0.835955 |
Target: 5'- aAGGAUGGCaaaCAGCGCGUgguacGCGUCGGAa- -3' miRNA: 3'- -UUCUGCCG---GUCGUGCG-----UGCGGUUUac -5' |
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22966 | 5' | -55.8 | NC_005137.2 | + | 98014 | 0.71 | 0.58653 |
Target: 5'- cAAG-UGGCCAGCGUGCACGUCGGGc- -3' miRNA: 3'- -UUCuGCCGGUCGUGCGUGCGGUUUac -5' |
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22966 | 5' | -55.8 | NC_005137.2 | + | 96687 | 0.67 | 0.817694 |
Target: 5'- cGGACGGCCguacacaaaaAGCggguucggcguugACGCAcCGCCAAAa- -3' miRNA: 3'- uUCUGCCGG----------UCG-------------UGCGU-GCGGUUUac -5' |
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22966 | 5' | -55.8 | NC_005137.2 | + | 96309 | 0.66 | 0.835955 |
Target: 5'- -cGACacGCCGGCGCGCAaguguggacUGCCGAAa- -3' miRNA: 3'- uuCUGc-CGGUCGUGCGU---------GCGGUUUac -5' |
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22966 | 5' | -55.8 | NC_005137.2 | + | 93516 | 0.66 | 0.844351 |
Target: 5'- -cGACGGCgCAaauGCACGCGuCgGCCGAAa- -3' miRNA: 3'- uuCUGCCG-GU---CGUGCGU-G-CGGUUUac -5' |
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22966 | 5' | -55.8 | NC_005137.2 | + | 93352 | 0.66 | 0.844351 |
Target: 5'- uGGACGGUguGUAuuuucgacUGCACGCCAc--- -3' miRNA: 3'- uUCUGCCGguCGU--------GCGUGCGGUuuac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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