Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23025 | 3' | -61.6 | NC_005178.1 | + | 31661 | 0.71 | 0.127456 |
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Target: 5'- gCCUGGACGG-GGCGGaCCagguccaGGCCGg -3' miRNA: 3'- gGGACUUGCCaCCGCC-GGag-----CCGGUu -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 30792 | 0.73 | 0.091116 |
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Target: 5'- gCCUGGcaGCGGUcGCGGCCUCgaccugGGCCGg -3' miRNA: 3'- gGGACU--UGCCAcCGCCGGAG------CCGGUu -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 18180 | 0.73 | 0.090859 |
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Target: 5'- aCCCUGGcgguucggcugcGCGGUGGCgucuaugGGCCg-GGCCAAa -3' miRNA: 3'- -GGGACU------------UGCCACCG-------CCGGagCCGGUU- -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 7078 | 0.74 | 0.074701 |
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Target: 5'- gCCCUGGGCGa-GGCGGugaCCUCGGUCGAg -3' miRNA: 3'- -GGGACUUGCcaCCGCC---GGAGCCGGUU- -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 4499 | 0.78 | 0.037403 |
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Target: 5'- aCCUGGGCGGUGGUGGCUUgGGUgGu -3' miRNA: 3'- gGGACUUGCCACCGCCGGAgCCGgUu -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 5728 | 0.79 | 0.034177 |
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Target: 5'- cCCCgGAGCagaucgccgcaucGGUGGCGGCCUCGGgCAc -3' miRNA: 3'- -GGGaCUUG-------------CCACCGCCGGAGCCgGUu -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 2894 | 0.81 | 0.023447 |
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Target: 5'- aCCCUGAugcUGGacaUGGCGcGCCUCGGCCAGg -3' miRNA: 3'- -GGGACUu--GCC---ACCGC-CGGAGCCGGUU- -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 16188 | 0.82 | 0.019096 |
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Target: 5'- aCCCUgcugcGAGCGGUGacCGGCCUCGGCCAu -3' miRNA: 3'- -GGGA-----CUUGCCACc-GCCGGAGCCGGUu -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 5335 | 1.07 | 0.000207 |
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Target: 5'- gCCCUGAACGGUGGCGGCCUCGGCCAAc -3' miRNA: 3'- -GGGACUUGCCACCGCCGGAGCCGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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