Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23025 | 3' | -61.6 | NC_005178.1 | + | 6698 | 0.68 | 0.207403 |
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Target: 5'- gCCUGGACcg-GGCGGCCUCacugcugaGCCAGg -3' miRNA: 3'- gGGACUUGccaCCGCCGGAGc-------CGGUU- -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 6579 | 0.69 | 0.175396 |
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Target: 5'- cCCUUGGccaaccgcaugcgcACGGUGGCGGCggugaccUGGCCGAa -3' miRNA: 3'- -GGGACU--------------UGCCACCGCCGga-----GCCGGUU- -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 5936 | 0.69 | 0.181619 |
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Target: 5'- gCgCUGGACGaugGGCGGCCgagGGCCGGu -3' miRNA: 3'- -GgGACUUGCca-CCGCCGGag-CCGGUU- -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 5728 | 0.79 | 0.034177 |
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Target: 5'- cCCCgGAGCagaucgccgcaucGGUGGCGGCCUCGGgCAc -3' miRNA: 3'- -GGGaCUUG-------------CCACCGCCGGAGCCgGUu -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 5335 | 1.07 | 0.000207 |
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Target: 5'- gCCCUGAACGGUGGCGGCCUCGGCCAAc -3' miRNA: 3'- -GGGACUUGCCACCGCCGGAGCCGGUU- -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 4603 | 0.66 | 0.2916 |
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Target: 5'- aCCaCUGGGCGGcguccggcucgaugGGCaGGCCggagcguugccgcUCGGCCAGc -3' miRNA: 3'- -GG-GACUUGCCa-------------CCG-CCGG-------------AGCCGGUU- -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 4499 | 0.78 | 0.037403 |
|
Target: 5'- aCCUGGGCGGUGGUGGCUUgGGUgGu -3' miRNA: 3'- gGGACUUGCCACCGCCGGAgCCGgUu -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 2894 | 0.81 | 0.023447 |
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Target: 5'- aCCCUGAugcUGGacaUGGCGcGCCUCGGCCAGg -3' miRNA: 3'- -GGGACUu--GCC---ACCGC-CGGAGCCGGUU- -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 2735 | 0.66 | 0.311657 |
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Target: 5'- gCCCUGGGCGa-GGUGGCCaccaaggCGGuCCGc -3' miRNA: 3'- -GGGACUUGCcaCCGCCGGa------GCC-GGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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