Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23025 | 3' | -61.6 | NC_005178.1 | + | 30792 | 0.73 | 0.091116 |
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Target: 5'- gCCUGGcaGCGGUcGCGGCCUCgaccugGGCCGg -3' miRNA: 3'- gGGACU--UGCCAcCGCCGGAG------CCGGUu -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 7410 | 0.7 | 0.158713 |
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Target: 5'- aCCUGAugGGcuuccaGcGCGacGCCUCGGCCGg -3' miRNA: 3'- gGGACUugCCa-----C-CGC--CGGAGCCGGUu -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 28157 | 0.68 | 0.19517 |
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Target: 5'- -gCUG-ACGGUcGGCGccucggcuguauccGCCUCGGCCGg -3' miRNA: 3'- ggGACuUGCCA-CCGC--------------CGGAGCCGGUu -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 12087 | 0.68 | 0.196729 |
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Target: 5'- gCCUGGugGaGUGGUcgcguggcgGGCCU-GGCCGAu -3' miRNA: 3'- gGGACUugC-CACCG---------CCGGAgCCGGUU- -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 6698 | 0.68 | 0.207403 |
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Target: 5'- gCCUGGACcg-GGCGGCCUCacugcugaGCCAGg -3' miRNA: 3'- gGGACUUGccaCCGCCGGAGc-------CGGUU- -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 28123 | 0.67 | 0.24876 |
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Target: 5'- gCCUGGGCGGUGGCa-CCgauggCGGCg-- -3' miRNA: 3'- gGGACUUGCCACCGccGGa----GCCGguu -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 12801 | 0.66 | 0.303362 |
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Target: 5'- uCCCUGGA-GGUcGCGGCCgccauguUCGGCg-- -3' miRNA: 3'- -GGGACUUgCCAcCGCCGG-------AGCCGguu -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 2735 | 0.66 | 0.311657 |
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Target: 5'- gCCCUGGGCGa-GGUGGCCaccaaggCGGuCCGc -3' miRNA: 3'- -GGGACUUGCcaCCGCCGGa------GCC-GGUu -5' |
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| 23025 | 3' | -61.6 | NC_005178.1 | + | 16188 | 0.82 | 0.019096 |
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Target: 5'- aCCCUgcugcGAGCGGUGacCGGCCUCGGCCAu -3' miRNA: 3'- -GGGA-----CUUGCCACc-GCCGGAGCCGGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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