Results 1 - 20 of 53 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23030 | 5' | -56.5 | NC_005178.1 | + | 19749 | 0.66 | 0.566514 |
Target: 5'- -aACCGU-CCGGCGCCaucUUcUGGCcaAGCc -3' miRNA: 3'- ggUGGCAuGGUCGCGG---AAcACCG--UCG- -5' |
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23030 | 5' | -56.5 | NC_005178.1 | + | 27944 | 0.66 | 0.555509 |
Target: 5'- gCC-CCGUugacCCAGuUGCCcUG-GGCGGCc -3' miRNA: 3'- -GGuGGCAu---GGUC-GCGGaACaCCGUCG- -5' |
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23030 | 5' | -56.5 | NC_005178.1 | + | 35726 | 0.66 | 0.544567 |
Target: 5'- uCCGCUccaggACguGCGCCUUGUccuGcGCAGUg -3' miRNA: 3'- -GGUGGca---UGguCGCGGAACA---C-CGUCG- -5' |
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23030 | 5' | -56.5 | NC_005178.1 | + | 22159 | 0.66 | 0.544567 |
Target: 5'- gCGCUGcUGCCGGUuacGgCUUcUGGCAGCu -3' miRNA: 3'- gGUGGC-AUGGUCG---CgGAAcACCGUCG- -5' |
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23030 | 5' | -56.5 | NC_005178.1 | + | 18489 | 0.66 | 0.544567 |
Target: 5'- uUACCGUugCAGCGCga----GCGGCg -3' miRNA: 3'- gGUGGCAugGUCGCGgaacacCGUCG- -5' |
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23030 | 5' | -56.5 | NC_005178.1 | + | 8755 | 0.66 | 0.544567 |
Target: 5'- -uGCCGUgggcgacuugGCCAGCGUgcg--GGCGGCg -3' miRNA: 3'- ggUGGCA----------UGGUCGCGgaacaCCGUCG- -5' |
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23030 | 5' | -56.5 | NC_005178.1 | + | 24578 | 0.66 | 0.544567 |
Target: 5'- -aGCCGgaUACCGGCGaCCUg--GGCcGCu -3' miRNA: 3'- ggUGGC--AUGGUCGC-GGAacaCCGuCG- -5' |
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23030 | 5' | -56.5 | NC_005178.1 | + | 11228 | 0.66 | 0.543476 |
Target: 5'- aCCAUCGUcgcguCCAGCGUCUUcggcccaauccugGUGGUuuaccuGCa -3' miRNA: 3'- -GGUGGCAu----GGUCGCGGAA-------------CACCGu-----CG- -5' |
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23030 | 5' | -56.5 | NC_005178.1 | + | 27729 | 0.66 | 0.533696 |
Target: 5'- gCGCUGUugC-GCGCCcaUGcccacGGCAGCg -3' miRNA: 3'- gGUGGCAugGuCGCGGa-ACa----CCGUCG- -5' |
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23030 | 5' | -56.5 | NC_005178.1 | + | 9325 | 0.66 | 0.533696 |
Target: 5'- aCGCCGUuuCCuGCGCCUgcgacgagUGccUGGCAcgGCg -3' miRNA: 3'- gGUGGCAu-GGuCGCGGA--------AC--ACCGU--CG- -5' |
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23030 | 5' | -56.5 | NC_005178.1 | + | 33577 | 0.66 | 0.533696 |
Target: 5'- gCGCUugaagaacuCCcGCGCCUgccgGUGGCAGUa -3' miRNA: 3'- gGUGGcau------GGuCGCGGAa---CACCGUCG- -5' |
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23030 | 5' | -56.5 | NC_005178.1 | + | 12429 | 0.66 | 0.522903 |
Target: 5'- gCCGCCGg--UAGUgGCCUg--GGCGGCa -3' miRNA: 3'- -GGUGGCaugGUCG-CGGAacaCCGUCG- -5' |
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23030 | 5' | -56.5 | NC_005178.1 | + | 20816 | 0.66 | 0.522903 |
Target: 5'- gCACCGagguacgcCCGGCGCUcugcugUUGggucagGGCGGCg -3' miRNA: 3'- gGUGGCau------GGUCGCGG------AACa-----CCGUCG- -5' |
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23030 | 5' | -56.5 | NC_005178.1 | + | 6050 | 0.66 | 0.522903 |
Target: 5'- -aGCCGUGaugaagaCGGUGCCgagggGUGGCcGCc -3' miRNA: 3'- ggUGGCAUg------GUCGCGGaa---CACCGuCG- -5' |
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23030 | 5' | -56.5 | NC_005178.1 | + | 13019 | 0.66 | 0.522903 |
Target: 5'- cCCGCCGUGaaGGUGCagcUGcUGGcCAGCc -3' miRNA: 3'- -GGUGGCAUggUCGCGga-AC-ACC-GUCG- -5' |
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23030 | 5' | -56.5 | NC_005178.1 | + | 3445 | 0.66 | 0.512195 |
Target: 5'- uCCACCGgccugGCCgAGUcgGCCcugGUGGUGGUc -3' miRNA: 3'- -GGUGGCa----UGG-UCG--CGGaa-CACCGUCG- -5' |
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23030 | 5' | -56.5 | NC_005178.1 | + | 31834 | 0.66 | 0.509 |
Target: 5'- aCGUCGagcUAUCAGCGCCUgcUGUccaccuuggacaacGGCAGCg -3' miRNA: 3'- gGUGGC---AUGGUCGCGGA--ACA--------------CCGUCG- -5' |
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23030 | 5' | -56.5 | NC_005178.1 | + | 9402 | 0.67 | 0.501578 |
Target: 5'- gCGCCGUGCCAG-GCaCUcGUcGCAGg -3' miRNA: 3'- gGUGGCAUGGUCgCG-GAaCAcCGUCg -5' |
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23030 | 5' | -56.5 | NC_005178.1 | + | 34647 | 0.67 | 0.49106 |
Target: 5'- cUCACCGacucgcccgGCCAGCuCCccgGUGGCAcGCc -3' miRNA: 3'- -GGUGGCa--------UGGUCGcGGaa-CACCGU-CG- -5' |
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23030 | 5' | -56.5 | NC_005178.1 | + | 28317 | 0.67 | 0.49106 |
Target: 5'- aCCAUUGgcggcgGCCAGCGag--GcGGCAGCg -3' miRNA: 3'- -GGUGGCa-----UGGUCGCggaaCaCCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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