Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23034 | 5' | -58.7 | NC_005178.1 | + | 20603 | 0.7 | 0.227567 |
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Target: 5'- gGcGAGCUGGACgGCCUgaucaaCAGCGCUauCCAg -3' miRNA: 3'- aC-CUUGACCUGgCGGA------GUUGCGG--GGU- -5' |
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| 23034 | 5' | -58.7 | NC_005178.1 | + | 25808 | 0.68 | 0.294771 |
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Target: 5'- aGGAGCaacgGGACCGCCUgcucggcacgucUGACGCCa-- -3' miRNA: 3'- aCCUUGa---CCUGGCGGA------------GUUGCGGggu -5' |
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| 23034 | 5' | -58.7 | NC_005178.1 | + | 17918 | 0.68 | 0.294771 |
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Target: 5'- aGGcgUuugUGGACCGCUcCAcguCGCCCCAg -3' miRNA: 3'- aCCuuG---ACCUGGCGGaGUu--GCGGGGU- -5' |
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| 23034 | 5' | -58.7 | NC_005178.1 | + | 3013 | 0.68 | 0.309938 |
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Target: 5'- cUGGAGCgcuuccUGGACCagGCCgagCGugGCGCCCUg -3' miRNA: 3'- -ACCUUG------ACCUGG--CGGa--GU--UGCGGGGu -5' |
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| 23034 | 5' | -58.7 | NC_005178.1 | + | 30938 | 0.67 | 0.358958 |
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Target: 5'- aGGAGCUGGGCaaCGUCgUCAGCaCCCa- -3' miRNA: 3'- aCCUUGACCUG--GCGG-AGUUGcGGGgu -5' |
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| 23034 | 5' | -58.7 | NC_005178.1 | + | 4505 | 0.67 | 0.367637 |
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Target: 5'- gUGcGAGCUGG-CCGagcggCAACGCUCCGg -3' miRNA: 3'- -AC-CUUGACCuGGCgga--GUUGCGGGGU- -5' |
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| 23034 | 5' | -58.7 | NC_005178.1 | + | 27083 | 0.67 | 0.367637 |
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Target: 5'- gGGAGCUGGACCGgagCAuUGCCgCGa -3' miRNA: 3'- aCCUUGACCUGGCggaGUuGCGGgGU- -5' |
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| 23034 | 5' | -58.7 | NC_005178.1 | + | 28153 | 0.67 | 0.380028 |
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Target: 5'- aGGGGCUgacggucggcgccucGGcuguauCCGCCUCGgccgGCGCCCUg -3' miRNA: 3'- aCCUUGA---------------CCu-----GGCGGAGU----UGCGGGGu -5' |
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| 23034 | 5' | -58.7 | NC_005178.1 | + | 5308 | 0.76 | 0.083386 |
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Target: 5'- cUGGAACUGG-CCGaCCUcCAGCGCCUgAa -3' miRNA: 3'- -ACCUUGACCuGGC-GGA-GUUGCGGGgU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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