Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23035 | 5' | -63 | NC_005178.1 | + | 28243 | 0.67 | 0.20543 |
Target: 5'- uCCAGgccgcUGCCGCCUc-GCuGGCCGCCg -3' miRNA: 3'- uGGUCa----GCGGCGGGuaCG-CCGGUGGa -5' |
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23035 | 5' | -63 | NC_005178.1 | + | 30234 | 0.67 | 0.1949 |
Target: 5'- aACUGGUCGUCGCaCCcauuUGaUGGCUACCa -3' miRNA: 3'- -UGGUCAGCGGCG-GGu---AC-GCCGGUGGa -5' |
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23035 | 5' | -63 | NC_005178.1 | + | 1525 | 0.67 | 0.194387 |
Target: 5'- gACCguuacgaGGUCGCCGCCCGcaUGuCGcGCCuguCCg -3' miRNA: 3'- -UGG-------UCAGCGGCGGGU--AC-GC-CGGu--GGa -5' |
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23035 | 5' | -63 | NC_005178.1 | + | 32219 | 0.73 | 0.068286 |
Target: 5'- gGCCAucGaCGCCGCCC-UG-GGCCGCCUg -3' miRNA: 3'- -UGGU--CaGCGGCGGGuACgCCGGUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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