miRNA: 3'- -UGGuCAGCGGCGGguaC-GCCgGUGGa -5'
miRNA: 3'- -UGGUC---AG-CGGCGGGUA--------CGC------CGGUGGa -5'
miRNA: 3'- uGGUCA--GCGG-CgGGUACGCCGg---UGGa -5'
miRNA: 3'- -UGGuCagCGGCGGGUaCgCCGGUGGa -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23035 | 5' | -63 | NC_005178.1 | + | 21198 | 0.66 | 0.240002 |
|
Target: 5'- gACC-GUCGCCGCCacgGaCGG-CACCg -3' miRNA: 3'- -UGGuCAGCGGCGGguaC-GCCgGUGGa -5' |
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| 23035 | 5' | -63 | NC_005178.1 | + | 5870 | 0.66 | 0.240002 |
|
Target: 5'- cACCGGcccUCgGCCGCCCAUcguccagcGCGagacgcGCCGCCa -3' miRNA: 3'- -UGGUC---AG-CGGCGGGUA--------CGC------CGGUGGa -5' |
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| 23035 | 5' | -63 | NC_005178.1 | + | 25871 | 0.66 | 0.246216 |
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Target: 5'- gUCAGUacCGCCaGgCCAUGCGGCaguuGCCg -3' miRNA: 3'- uGGUCA--GCGG-CgGGUACGCCGg---UGGa -5' |
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| 23035 | 5' | -63 | NC_005178.1 | + | 7986 | 0.66 | 0.252562 |
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Target: 5'- aACCcGagGCCGUCCA-GgGGCUGCCg -3' miRNA: 3'- -UGGuCagCGGCGGGUaCgCCGGUGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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