Results 1 - 20 of 56 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23044 | 3' | -62.4 | NC_005178.1 | + | 2905 | 0.67 | 0.227967 |
Target: 5'- --gGACaUGGCGCGCCucgGCCaggUCGaGCCGc -3' miRNA: 3'- cuaCUG-GCCGCGCGG---CGGa--AGC-CGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 3808 | 0.75 | 0.054339 |
Target: 5'- cGAUGGcCCGGCGCGUCaGCUUgaaggUGGCCAg -3' miRNA: 3'- -CUACU-GGCCGCGCGG-CGGAa----GCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 3916 | 0.66 | 0.286326 |
Target: 5'- cGAUG-CgCGGCaaGCUGCUgugCGGCCGg -3' miRNA: 3'- -CUACuG-GCCGcgCGGCGGaa-GCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 4574 | 0.75 | 0.062692 |
Target: 5'- --aGGCCGGaGCGuuGCCgcUCGGCCAg -3' miRNA: 3'- cuaCUGGCCgCGCggCGGa-AGCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 5009 | 0.73 | 0.076496 |
Target: 5'- cGAUGAUCagcgGGCG-GCCGCUggCGGCCAg -3' miRNA: 3'- -CUACUGG----CCGCgCGGCGGaaGCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 5295 | 0.66 | 0.259043 |
Target: 5'- --gGGCCGGCGCGa-GCUggaacUGGCCGa -3' miRNA: 3'- cuaCUGGCCGCGCggCGGaa---GCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 7413 | 0.69 | 0.166105 |
Target: 5'- uGAUGGgcuuCCaGCGCGaCGCC-UCGGCCGg -3' miRNA: 3'- -CUACU----GGcCGCGCgGCGGaAGCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 7880 | 0.7 | 0.137382 |
Target: 5'- cGUGACUGuGCGCGgCGCCgaugCGGUCc -3' miRNA: 3'- cUACUGGC-CGCGCgGCGGaa--GCCGGu -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 9521 | 0.66 | 0.252562 |
Target: 5'- --aGACCGccaGCGCCGCCUggcgCGggaacGCCAa -3' miRNA: 3'- cuaCUGGCcg-CGCGGCGGAa---GC-----CGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 9661 | 0.66 | 0.286326 |
Target: 5'- --cGGCCGGUG-GCuuCGCCgagcCGGCCGa -3' miRNA: 3'- cuaCUGGCCGCgCG--GCGGaa--GCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 9723 | 0.67 | 0.216447 |
Target: 5'- --cGGCCGGCucgGCGaaGCCacCGGCCGc -3' miRNA: 3'- cuaCUGGCCG---CGCggCGGaaGCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 10249 | 0.68 | 0.200105 |
Target: 5'- cAUGGCCGGaCGCauCgGCCUgcCGGCCAc -3' miRNA: 3'- cUACUGGCC-GCGc-GgCGGAa-GCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 10636 | 0.66 | 0.246216 |
Target: 5'- ---aGCCGGgGCGCaaCGCCc-CGGCCGg -3' miRNA: 3'- cuacUGGCCgCGCG--GCGGaaGCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 10941 | 0.66 | 0.265658 |
Target: 5'- --cGAuCCGGCGCGgcCCGCCUcCuGCCc -3' miRNA: 3'- cuaCU-GGCCGCGC--GGCGGAaGcCGGu -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 11100 | 0.68 | 0.179993 |
Target: 5'- --cGGCCGGCaGCGCCGCgCUgcugaaaauguUUGGCa- -3' miRNA: 3'- cuaCUGGCCG-CGCGGCG-GA-----------AGCCGgu -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 12119 | 0.75 | 0.062692 |
Target: 5'- --cGAUgGGCGCGCCGCCUgc-GCCGa -3' miRNA: 3'- cuaCUGgCCGCGCGGCGGAagcCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 12172 | 0.66 | 0.265658 |
Target: 5'- --aGG-CGGCGCGCC-Ca-UCGGCCAg -3' miRNA: 3'- cuaCUgGCCGCGCGGcGgaAGCCGGU- -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 12660 | 0.75 | 0.055918 |
Target: 5'- --cGGCCGGCGCGCUGgCgggUGGCCu -3' miRNA: 3'- cuaCUGGCCGCGCGGCgGaa-GCCGGu -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 12791 | 0.67 | 0.209229 |
Target: 5'- --cGACCGGCagucccuggaggucGCgGCCGCCaugUUCGGCg- -3' miRNA: 3'- cuaCUGGCCG--------------CG-CGGCGG---AAGCCGgu -5' |
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23044 | 3' | -62.4 | NC_005178.1 | + | 12935 | 0.66 | 0.27241 |
Target: 5'- --gGACgUGGCGCGCCagguGCUggcCGGCCu -3' miRNA: 3'- cuaCUG-GCCGCGCGG----CGGaa-GCCGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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