Results 1 - 20 of 48 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23049 | 5' | -58.2 | NC_005178.1 | + | 9656 | 0.66 | 0.438638 |
Target: 5'- ---cUGCgCGGCCGguggcuucgccgagcCGGCCGaGGCGgUCa -3' miRNA: 3'- cuuuACG-GCCGGU---------------GCCGGUaCCGC-AG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 7068 | 0.66 | 0.434692 |
Target: 5'- uGAGUGcCCGGcCCugGGCgA-GGCGg- -3' miRNA: 3'- cUUUAC-GGCC-GGugCCGgUaCCGCag -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 3946 | 0.66 | 0.434692 |
Target: 5'- --uGUGgCGGCgGCGGCC--GGUGUUc -3' miRNA: 3'- cuuUACgGCCGgUGCCGGuaCCGCAG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 28771 | 0.66 | 0.434692 |
Target: 5'- ---uUGCCGGCCcacauguCGGCCGccaucUGGUcgggGUCu -3' miRNA: 3'- cuuuACGGCCGGu------GCCGGU-----ACCG----CAG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 6871 | 0.66 | 0.434692 |
Target: 5'- cGAucUGUCGGCCugGGgCGa-GCGUCc -3' miRNA: 3'- -CUuuACGGCCGGugCCgGUacCGCAG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 4492 | 0.66 | 0.424917 |
Target: 5'- -cGGUGgUGGCUugGGUgGUGGCugcgGUCg -3' miRNA: 3'- cuUUACgGCCGGugCCGgUACCG----CAG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 34738 | 0.66 | 0.415274 |
Target: 5'- gGAAcgGCUGGCCAggguucaGGCUAcgGGCG-Cg -3' miRNA: 3'- -CUUuaCGGCCGGUg------CCGGUa-CCGCaG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 31500 | 0.66 | 0.409554 |
Target: 5'- ----gGCCGGCCucgacuuccgcgacgGCGGUC-UGGCGa- -3' miRNA: 3'- cuuuaCGGCCGG---------------UGCCGGuACCGCag -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 35701 | 0.66 | 0.405767 |
Target: 5'- ----cGCUGGCCGaGGaCAUGGCGg- -3' miRNA: 3'- cuuuaCGGCCGGUgCCgGUACCGCag -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 22910 | 0.66 | 0.405767 |
Target: 5'- ----cGCCGGaUCgAUGGUCGUGGCGg- -3' miRNA: 3'- cuuuaCGGCC-GG-UGCCGGUACCGCag -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 25698 | 0.66 | 0.405767 |
Target: 5'- ---cUGCCGGUCGaacuCGGCgGUGGCu-- -3' miRNA: 3'- cuuuACGGCCGGU----GCCGgUACCGcag -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 8920 | 0.66 | 0.396399 |
Target: 5'- ----aGCCcaGGUuuCGCGGUCgGUGGCGUCg -3' miRNA: 3'- cuuuaCGG--CCG--GUGCCGG-UACCGCAG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 5917 | 0.66 | 0.396399 |
Target: 5'- cGAggGCCGGUguaGCGGCCuggcuugagGGCGUa -3' miRNA: 3'- cUUuaCGGCCGg--UGCCGGua-------CCGCAg -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 21281 | 0.66 | 0.395469 |
Target: 5'- ----gGCCGGCCggggaGCGGguuucggugccguCCGUGGCGg- -3' miRNA: 3'- cuuuaCGGCCGG-----UGCC-------------GGUACCGCag -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 37048 | 0.67 | 0.38717 |
Target: 5'- aGAGuggGCCGGCUcgcuggacgagGCGGCagcacucgcUGGCGUCc -3' miRNA: 3'- -CUUua-CGGCCGG-----------UGCCGgu-------ACCGCAG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 14276 | 0.67 | 0.381701 |
Target: 5'- ---uUGCCGGCgcccugggggaagucCAUGGCCA-GGCG-Cg -3' miRNA: 3'- cuuuACGGCCG---------------GUGCCGGUaCCGCaG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 23247 | 0.67 | 0.378084 |
Target: 5'- cGAGGUGCCcGGCCuucaggACGuuUcgGGCGUCc -3' miRNA: 3'- -CUUUACGG-CCGG------UGCcgGuaCCGCAG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 23445 | 0.67 | 0.378084 |
Target: 5'- ----cGCCcagGGCgACGGCCAgGGCGcCc -3' miRNA: 3'- cuuuaCGG---CCGgUGCCGGUaCCGCaG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 32295 | 0.67 | 0.377184 |
Target: 5'- ----gGUCGGCUcgaccagGCGGCCcagggcGGCGUCg -3' miRNA: 3'- cuuuaCGGCCGG-------UGCCGGua----CCGCAG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 31915 | 0.67 | 0.360347 |
Target: 5'- ---cUGCCGGUCAuCGGCCAUcgcugaucGGCa-- -3' miRNA: 3'- cuuuACGGCCGGU-GCCGGUA--------CCGcag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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