Results 1 - 20 of 48 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23049 | 5' | -58.2 | NC_005178.1 | + | 3428 | 0.69 | 0.287887 |
Target: 5'- gGAAggGCCGGCCGugcaucgggugcUGGaCCucGGCGUCg -3' miRNA: 3'- -CUUuaCGGCCGGU------------GCC-GGuaCCGCAG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 3823 | 0.69 | 0.259556 |
Target: 5'- -uGGUGCCgcccuuGGCgAUGGCCcgGcGCGUCa -3' miRNA: 3'- cuUUACGG------CCGgUGCCGGuaC-CGCAG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 3946 | 0.66 | 0.434692 |
Target: 5'- --uGUGgCGGCgGCGGCC--GGUGUUc -3' miRNA: 3'- cuuUACgGCCGgUGCCGGuaCCGCAG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 4492 | 0.66 | 0.424917 |
Target: 5'- -cGGUGgUGGCUugGGUgGUGGCugcgGUCg -3' miRNA: 3'- cuUUACgGCCGGugCCGgUACCG----CAG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 4718 | 0.69 | 0.280584 |
Target: 5'- -cGAUGUCGGCCAugccauUGGCCAguugGGUGg- -3' miRNA: 3'- cuUUACGGCCGGU------GCCGGUa---CCGCag -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 5917 | 0.66 | 0.396399 |
Target: 5'- cGAggGCCGGUguaGCGGCCuggcuugagGGCGUa -3' miRNA: 3'- cUUuaCGGCCGg--UGCCGGua-------CCGCAg -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 6871 | 0.66 | 0.434692 |
Target: 5'- cGAucUGUCGGCCugGGgCGa-GCGUCc -3' miRNA: 3'- -CUuuACGGCCGGugCCgGUacCGCAG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 7068 | 0.66 | 0.434692 |
Target: 5'- uGAGUGcCCGGcCCugGGCgA-GGCGg- -3' miRNA: 3'- cUUUAC-GGCC-GGugCCGgUaCCGCag -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 8920 | 0.66 | 0.396399 |
Target: 5'- ----aGCCcaGGUuuCGCGGUCgGUGGCGUCg -3' miRNA: 3'- cuuuaCGG--CCG--GUGCCGG-UACCGCAG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 9656 | 0.66 | 0.438638 |
Target: 5'- ---cUGCgCGGCCGguggcuucgccgagcCGGCCGaGGCGgUCa -3' miRNA: 3'- cuuuACG-GCCGGU---------------GCCGGUaCCGC-AG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 10064 | 0.73 | 0.142353 |
Target: 5'- cGGuuUGCCGGUUuuGCGGCacuuuUGGCGUCg -3' miRNA: 3'- -CUuuACGGCCGG--UGCCGgu---ACCGCAG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 10268 | 0.7 | 0.22739 |
Target: 5'- ---cUGCCGGCCACGcUgGUGGUaGUCg -3' miRNA: 3'- cuuuACGGCCGGUGCcGgUACCG-CAG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 11215 | 0.68 | 0.334848 |
Target: 5'- aGGAA-GCCcagGGCgGCGGCCAgGGCGcCc -3' miRNA: 3'- -CUUUaCGG---CCGgUGCCGGUaCCGCaG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 11747 | 0.68 | 0.302942 |
Target: 5'- ----cGCauauGCCACGGCCGUGGCc-- -3' miRNA: 3'- cuuuaCGgc--CGGUGCCGGUACCGcag -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 11810 | 0.76 | 0.082402 |
Target: 5'- ----gGCUcgGGCCACGGCCGUGGCa-- -3' miRNA: 3'- cuuuaCGG--CCGGUGCCGGUACCGcag -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 12840 | 0.72 | 0.178007 |
Target: 5'- gGGAcUGCCGGUCgaggacguaggcGCGGCgCAgGGCGUCg -3' miRNA: 3'- -CUUuACGGCCGG------------UGCCG-GUaCCGCAG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 14276 | 0.67 | 0.381701 |
Target: 5'- ---uUGCCGGCgcccugggggaagucCAUGGCCA-GGCG-Cg -3' miRNA: 3'- cuuuACGGCCG---------------GUGCCGGUaCCGCaG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 15793 | 0.73 | 0.154886 |
Target: 5'- ----cGUCGGCCuucucuGCGGCCGaGGCGUUg -3' miRNA: 3'- cuuuaCGGCCGG------UGCCGGUaCCGCAG- -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 15828 | 0.71 | 0.198663 |
Target: 5'- ----cGUCGGCCACGGCUAUaGCGc- -3' miRNA: 3'- cuuuaCGGCCGGUGCCGGUAcCGCag -5' |
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23049 | 5' | -58.2 | NC_005178.1 | + | 16201 | 0.69 | 0.259556 |
Target: 5'- -cGGUGaCCGGCCuCGGCCAUGccgcaGCGg- -3' miRNA: 3'- cuUUAC-GGCCGGuGCCGGUAC-----CGCag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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