Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 23055 | 3' | -53.5 | NC_005178.1 | + | 35604 | 0.72 | 0.37291 |
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Target: 5'- gGCCCGGAAGCug-GGgCGACggugacgcuCCAGGu -3' miRNA: 3'- gUGGGCCUUCGuuaUUgGCUG---------GGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 33907 | 0.72 | 0.390376 |
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Target: 5'- gCGCCUGGcGGCGAUGcucggcggccgguGCCGGCuuuCCGGGg -3' miRNA: 3'- -GUGGGCCuUCGUUAU-------------UGGCUG---GGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 23465 | 0.72 | 0.37291 |
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Target: 5'- gCGCCCGGc-GCGA-AGCCGGCCCGc- -3' miRNA: 3'- -GUGGGCCuuCGUUaUUGGCUGGGUcc -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 7512 | 0.72 | 0.372005 |
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Target: 5'- uGCCCuGGAAGCGggugauguaacggGUGcuGCCGGCCgAGGc -3' miRNA: 3'- gUGGG-CCUUCGU-------------UAU--UGGCUGGgUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 8986 | 0.73 | 0.346433 |
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Target: 5'- aCACCCccagcgcacGGAAGCcgggccGAUccuGCUGGCCCAGGg -3' miRNA: 3'- -GUGGG---------CCUUCG------UUAu--UGGCUGGGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 16190 | 0.73 | 0.337911 |
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Target: 5'- cCugCUGcGAGCGGUGACCGGCCUcGGc -3' miRNA: 3'- -GugGGCcUUCGUUAUUGGCUGGGuCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 22066 | 0.73 | 0.329543 |
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Target: 5'- aGCCCaGGAAGCA---GCCGGaauucguuuCCCAGGu -3' miRNA: 3'- gUGGG-CCUUCGUuauUGGCU---------GGGUCC- -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 29013 | 0.78 | 0.150436 |
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Target: 5'- uCACCUGGcgcacGGCGAUGGCCGugCCGGa -3' miRNA: 3'- -GUGGGCCu----UCGUUAUUGGCugGGUCc -5' |
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| 23055 | 3' | -53.5 | NC_005178.1 | + | 30720 | 1.09 | 0.00096 |
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Target: 5'- uCACCCGGAAGCAAUAACCGACCCAGGc -3' miRNA: 3'- -GUGGGCCUUCGUUAUUGGCUGGGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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