Results 21 - 40 of 69 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23055 | 3' | -53.5 | NC_005178.1 | + | 34638 | 0.69 | 0.513038 |
Target: 5'- uCGCCCGGccAGCuccccGGUGGCaCG-CCCAGGa -3' miRNA: 3'- -GUGGGCCu-UCG-----UUAUUG-GCuGGGUCC- -5' |
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23055 | 3' | -53.5 | NC_005178.1 | + | 8886 | 0.69 | 0.513038 |
Target: 5'- aCACCCGGcacgcgccGGGCGAcuuucuuGCCGACCUGGu -3' miRNA: 3'- -GUGGGCC--------UUCGUUau-----UGGCUGGGUCc -5' |
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23055 | 3' | -53.5 | NC_005178.1 | + | 16787 | 0.69 | 0.52283 |
Target: 5'- aGCgCUGGAccaggugcuggccAGCcu--GCCGGCCCAGGa -3' miRNA: 3'- gUG-GGCCU-------------UCGuuauUGGCUGGGUCC- -5' |
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23055 | 3' | -53.5 | NC_005178.1 | + | 17703 | 0.69 | 0.534894 |
Target: 5'- gAUUCGGGAGCAGaccuUGACC-ACCCuGGa -3' miRNA: 3'- gUGGGCCUUCGUU----AUUGGcUGGGuCC- -5' |
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23055 | 3' | -53.5 | NC_005178.1 | + | 16797 | 0.69 | 0.510872 |
Target: 5'- uCACCUGGGccagcgcAGCGAUGcgaggaccagggcGCCGGCUCAGu -3' miRNA: 3'- -GUGGGCCU-------UCGUUAU-------------UGGCUGGGUCc -5' |
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23055 | 3' | -53.5 | NC_005178.1 | + | 28157 | 0.68 | 0.629392 |
Target: 5'- aACCCGGaAAGCAGagacgccccUAGCUGGCgggucgccauccuggCCAGGu -3' miRNA: 3'- gUGGGCC-UUCGUU---------AUUGGCUG---------------GGUCC- -5' |
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23055 | 3' | -53.5 | NC_005178.1 | + | 13152 | 0.68 | 0.568256 |
Target: 5'- uCAUCCGuGAGCGGUucCCGcagcacGCCCAGGc -3' miRNA: 3'- -GUGGGCcUUCGUUAuuGGC------UGGGUCC- -5' |
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23055 | 3' | -53.5 | NC_005178.1 | + | 8904 | 0.68 | 0.579499 |
Target: 5'- gACCCuGGGccAGCAG-GAUCGGCCCGGc -3' miRNA: 3'- gUGGG-CCU--UCGUUaUUGGCUGGGUCc -5' |
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23055 | 3' | -53.5 | NC_005178.1 | + | 1453 | 0.68 | 0.579499 |
Target: 5'- aUACCUGGugaguGCAAcuacGCCG-CCCAGGu -3' miRNA: 3'- -GUGGGCCuu---CGUUau--UGGCuGGGUCC- -5' |
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23055 | 3' | -53.5 | NC_005178.1 | + | 8977 | 0.68 | 0.590788 |
Target: 5'- uCACCCGGAcagagcuacGGCGccguagacgucgAUAGCCGACuCCGc- -3' miRNA: 3'- -GUGGGCCU---------UCGU------------UAUUGGCUG-GGUcc -5' |
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23055 | 3' | -53.5 | NC_005178.1 | + | 25413 | 0.68 | 0.590788 |
Target: 5'- gGCCCGGAucaaGGCGAUGgucGCCGccuCCCugcaacagcGGGa -3' miRNA: 3'- gUGGGCCU----UCGUUAU---UGGCu--GGG---------UCC- -5' |
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23055 | 3' | -53.5 | NC_005178.1 | + | 26776 | 0.68 | 0.590788 |
Target: 5'- aCGCCUGG-AGCA---ACUGACCaAGGg -3' miRNA: 3'- -GUGGGCCuUCGUuauUGGCUGGgUCC- -5' |
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23055 | 3' | -53.5 | NC_005178.1 | + | 17843 | 0.68 | 0.602115 |
Target: 5'- gCACCCuGGGAGCGccuGCCG-CCCucGGc -3' miRNA: 3'- -GUGGG-CCUUCGUuauUGGCuGGGu-CC- -5' |
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23055 | 3' | -53.5 | NC_005178.1 | + | 27155 | 0.68 | 0.579499 |
Target: 5'- cCGCCUGGAucgcGGCAAUGcuCCGGUCCAGc -3' miRNA: 3'- -GUGGGCCU----UCGUUAUu-GGCUGGGUCc -5' |
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23055 | 3' | -53.5 | NC_005178.1 | + | 7965 | 0.68 | 0.621428 |
Target: 5'- aACaaGGAAGCGAUccucaagaacccgaGGCCG-UCCAGGg -3' miRNA: 3'- gUGggCCUUCGUUA--------------UUGGCuGGGUCC- -5' |
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23055 | 3' | -53.5 | NC_005178.1 | + | 28287 | 0.68 | 0.602115 |
Target: 5'- gGCCUGGAuAGCGGcggcGCCG-CUCAGGa -3' miRNA: 3'- gUGGGCCU-UCGUUau--UGGCuGGGUCC- -5' |
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23055 | 3' | -53.5 | NC_005178.1 | + | 20451 | 0.68 | 0.602115 |
Target: 5'- uCGCCUGGgcGUccagGGCCGGCUuCAGGg -3' miRNA: 3'- -GUGGGCCuuCGuua-UUGGCUGG-GUCC- -5' |
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23055 | 3' | -53.5 | NC_005178.1 | + | 9660 | 0.67 | 0.63622 |
Target: 5'- gCGgCCGGuGGCuucgccgAGCCGGCCgAGGc -3' miRNA: 3'- -GUgGGCCuUCGuua----UUGGCUGGgUCC- -5' |
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23055 | 3' | -53.5 | NC_005178.1 | + | 30888 | 0.67 | 0.681581 |
Target: 5'- gCGCCCuGGAAGaCAAcuauuacAACCGGCUguGGc -3' miRNA: 3'- -GUGGG-CCUUC-GUUa------UUGGCUGGguCC- -5' |
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23055 | 3' | -53.5 | NC_005178.1 | + | 26910 | 0.67 | 0.681581 |
Target: 5'- aGCgCgGGAAGCAAUucACCGAUgcccuUCAGGa -3' miRNA: 3'- gUG-GgCCUUCGUUAu-UGGCUG-----GGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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