Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23057 | 3' | -60.4 | NC_005178.1 | + | 35160 | 0.66 | 0.342082 |
Target: 5'- aGCCGGCuGGaggacGUCACUGUCggccaGGCCg -3' miRNA: 3'- -CGGCCG-CCgc---CAGUGACGGaa---CUGGa -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 3468 | 0.67 | 0.310431 |
Target: 5'- cCUGGUGGUGGUCGaUGCCcuacgUGACg- -3' miRNA: 3'- cGGCCGCCGCCAGUgACGGa----ACUGga -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 26089 | 0.67 | 0.295461 |
Target: 5'- cCCGaGgGGCGGaCGCUGCCgcagcgucGGCCa -3' miRNA: 3'- cGGC-CgCCGCCaGUGACGGaa------CUGGa -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 12086 | 0.68 | 0.274069 |
Target: 5'- cGCCuGGUGGaguGGUCGCguggcggGCCU-GGCCg -3' miRNA: 3'- -CGG-CCGCCg--CCAGUGa------CGGAaCUGGa -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 12414 | 0.68 | 0.2475 |
Target: 5'- cCCGGUGGCGGUgACUGa--UGACa- -3' miRNA: 3'- cGGCCGCCGCCAgUGACggaACUGga -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 8778 | 0.68 | 0.2475 |
Target: 5'- uGCgGGCGGCGGUCuuUGCa---ACCg -3' miRNA: 3'- -CGgCCGCCGCCAGugACGgaacUGGa -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 9679 | 0.69 | 0.235034 |
Target: 5'- aGCCGGCcgaGGCGGUCACcuUGCUccuacacaacgUUG-CCg -3' miRNA: 3'- -CGGCCG---CCGCCAGUG--ACGG-----------AACuGGa -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 26181 | 0.7 | 0.190357 |
Target: 5'- gGCgCGGCGGUGGcCGCUGCgggcgUGGCgCUg -3' miRNA: 3'- -CG-GCCGCCGCCaGUGACGga---ACUG-GA- -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 25898 | 0.71 | 0.153314 |
Target: 5'- uGCCGGCGcagaucaccgacGUGGUCACcaGCCU-GGCCa -3' miRNA: 3'- -CGGCCGC------------CGCCAGUGa-CGGAaCUGGa -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 28293 | 0.71 | 0.153314 |
Target: 5'- aCCGGCGcuGCGGgaGCUGCCggGGCCg -3' miRNA: 3'- cGGCCGC--CGCCagUGACGGaaCUGGa -5' |
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23057 | 3' | -60.4 | NC_005178.1 | + | 12653 | 0.72 | 0.141193 |
Target: 5'- gGCuCGGCGGcCGGcgCGCUGgCgggUGGCCUg -3' miRNA: 3'- -CG-GCCGCC-GCCa-GUGACgGa--ACUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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