Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 23070 | 3' | -54.8 | NC_005178.1 | + | 27292 | 0.7 | 0.379152 |
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Target: 5'- --cCAGcGUGGcCGGCagGCCGAUGCg -3' miRNA: 3'- ucuGUCuCGCCaGUCGaaCGGCUACG- -5' |
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| 23070 | 3' | -54.8 | NC_005178.1 | + | 13153 | 0.7 | 0.379152 |
|
Target: 5'- -cGCAGGGCaacGGUCGGCUgcgGCgCGGUGa -3' miRNA: 3'- ucUGUCUCG---CCAGUCGAa--CG-GCUACg -5' |
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| 23070 | 3' | -54.8 | NC_005178.1 | + | 6437 | 0.71 | 0.310449 |
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Target: 5'- cGGGCGG-GCcGUCGGU--GCCGAUGCu -3' miRNA: 3'- -UCUGUCuCGcCAGUCGaaCGGCUACG- -5' |
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| 23070 | 3' | -54.8 | NC_005178.1 | + | 36202 | 0.73 | 0.238446 |
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Target: 5'- uGGACGGAGCuGGUaGGCgggaugGCCGAcGCg -3' miRNA: 3'- -UCUGUCUCG-CCAgUCGaa----CGGCUaCG- -5' |
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| 23070 | 3' | -54.8 | NC_005178.1 | + | 5889 | 0.73 | 0.251638 |
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Target: 5'- uAGGCuGuuCGGUCAGC-UGCgCGGUGCg -3' miRNA: 3'- -UCUGuCucGCCAGUCGaACG-GCUACG- -5' |
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| 23070 | 3' | -54.8 | NC_005178.1 | + | 15291 | 0.74 | 0.202317 |
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Target: 5'- uGGCGGgccagccuGGCGGUCGGCUUGgCGGuaguUGCg -3' miRNA: 3'- uCUGUC--------UCGCCAGUCGAACgGCU----ACG- -5' |
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| 23070 | 3' | -54.8 | NC_005178.1 | + | 13 | 1.11 | 0.000451 |
|
Target: 5'- cAGACAGAGCGGUCAGCUUGCCGAUGCc -3' miRNA: 3'- -UCUGUCUCGCCAGUCGAACGGCUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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