Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23070 | 3' | -54.8 | NC_005178.1 | + | 16670 | 0.66 | 0.577037 |
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Target: 5'- uGGCGaGGCGGUCcugGGCgaucagGCCGgcGCa -3' miRNA: 3'- uCUGUcUCGCCAG---UCGaa----CGGCuaCG- -5' |
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| 23070 | 3' | -54.8 | NC_005178.1 | + | 23227 | 0.66 | 0.584968 |
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Target: 5'- aGGACGGuGCGGgccgugUCGaaggccgcgcauccGCUUGCCGGaGCc -3' miRNA: 3'- -UCUGUCuCGCC------AGU--------------CGAACGGCUaCG- -5' |
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| 23070 | 3' | -54.8 | NC_005178.1 | + | 17180 | 0.66 | 0.588374 |
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Target: 5'- cGGcuuCAGGGCGGUCAGg--GCCGcaaugGCc -3' miRNA: 3'- -UCu--GUCUCGCCAGUCgaaCGGCua---CG- -5' |
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| 23070 | 3' | -54.8 | NC_005178.1 | + | 18137 | 0.66 | 0.588374 |
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Target: 5'- gAGGCcGAGCaGcUCGGCUgcgcgccguugGCUGAUGCc -3' miRNA: 3'- -UCUGuCUCGcC-AGUCGAa----------CGGCUACG- -5' |
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| 23070 | 3' | -54.8 | NC_005178.1 | + | 13511 | 0.66 | 0.599751 |
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Target: 5'- uGACGGuGGCGGUC---UUGCCGAuagggaagcUGCg -3' miRNA: 3'- uCUGUC-UCGCCAGucgAACGGCU---------ACG- -5' |
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| 23070 | 3' | -54.8 | NC_005178.1 | + | 13032 | 0.66 | 0.599751 |
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Target: 5'- -uGCAGcuGCuGGcCAGCcugGCCGAUGCc -3' miRNA: 3'- ucUGUCu-CG-CCaGUCGaa-CGGCUACG- -5' |
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| 23070 | 3' | -54.8 | NC_005178.1 | + | 28125 | 0.66 | 0.634019 |
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Target: 5'- uAGGcCGGGGCGGUUucGCUcagUGgaCGGUGCg -3' miRNA: 3'- -UCU-GUCUCGCCAGu-CGA---ACg-GCUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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