miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23070 3' -54.8 NC_005178.1 + 5954 0.69 0.417126
Target:  5'- uGGACGGGGCGGacCAGgUccagGCCGGcgGCg -3'
miRNA:   3'- -UCUGUCUCGCCa-GUCgAa---CGGCUa-CG- -5'
23070 3' -54.8 NC_005178.1 + 27292 0.7 0.379152
Target:  5'- --cCAGcGUGGcCGGCagGCCGAUGCg -3'
miRNA:   3'- ucuGUCuCGCCaGUCGaaCGGCUACG- -5'
23070 3' -54.8 NC_005178.1 + 13153 0.7 0.379152
Target:  5'- -cGCAGGGCaacGGUCGGCUgcgGCgCGGUGa -3'
miRNA:   3'- ucUGUCUCG---CCAGUCGAa--CG-GCUACg -5'
23070 3' -54.8 NC_005178.1 + 6437 0.71 0.310449
Target:  5'- cGGGCGG-GCcGUCGGU--GCCGAUGCu -3'
miRNA:   3'- -UCUGUCuCGcCAGUCGaaCGGCUACG- -5'
23070 3' -54.8 NC_005178.1 + 5889 0.73 0.251638
Target:  5'- uAGGCuGuuCGGUCAGC-UGCgCGGUGCg -3'
miRNA:   3'- -UCUGuCucGCCAGUCGaACG-GCUACG- -5'
23070 3' -54.8 NC_005178.1 + 15291 0.74 0.202317
Target:  5'- uGGCGGgccagccuGGCGGUCGGCUUGgCGGuaguUGCg -3'
miRNA:   3'- uCUGUC--------UCGCCAGUCGAACgGCU----ACG- -5'
23070 3' -54.8 NC_005178.1 + 13 1.11 0.000451
Target:  5'- cAGACAGAGCGGUCAGCUUGCCGAUGCc -3'
miRNA:   3'- -UCUGUCUCGCCAGUCGAACGGCUACG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.