Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23070 | 3' | -54.8 | NC_005178.1 | + | 30270 | 0.67 | 0.532266 |
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Target: 5'- cGGuACAGcGGCGGcggCAGCgUGCUGcgGCu -3' miRNA: 3'- -UC-UGUC-UCGCCa--GUCGaACGGCuaCG- -5' |
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| 23070 | 3' | -54.8 | NC_005178.1 | + | 30558 | 0.68 | 0.510344 |
|
Target: 5'- cGACGGguAGCGGUUGGCgagGauGGUGCu -3' miRNA: 3'- uCUGUC--UCGCCAGUCGaa-CggCUACG- -5' |
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| 23070 | 3' | -54.8 | NC_005178.1 | + | 32917 | 0.68 | 0.510344 |
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Target: 5'- -aGCGGcGUGGUCgccGGCaUGCCGAUGg -3' miRNA: 3'- ucUGUCuCGCCAG---UCGaACGGCUACg -5' |
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| 23070 | 3' | -54.8 | NC_005178.1 | + | 33086 | 0.68 | 0.488807 |
|
Target: 5'- uGGGCgGGAGCGGUCGGCagggUGaaGAccuggGCg -3' miRNA: 3'- -UCUG-UCUCGCCAGUCGa---ACggCUa----CG- -5' |
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| 23070 | 3' | -54.8 | NC_005178.1 | + | 33804 | 0.67 | 0.543355 |
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Target: 5'- cGAUGGcccGGCGcGUCAGCUUGaaGGUGg -3' miRNA: 3'- uCUGUC---UCGC-CAGUCGAACggCUACg -5' |
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| 23070 | 3' | -54.8 | NC_005178.1 | + | 34683 | 0.68 | 0.478199 |
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Target: 5'- -aAUGGAGCGuuucCGGCUguaGCCGGUGCg -3' miRNA: 3'- ucUGUCUCGCca--GUCGAa--CGGCUACG- -5' |
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| 23070 | 3' | -54.8 | NC_005178.1 | + | 36202 | 0.73 | 0.238446 |
|
Target: 5'- uGGACGGAGCuGGUaGGCgggaugGCCGAcGCg -3' miRNA: 3'- -UCUGUCUCG-CCAgUCGaa----CGGCUaCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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