Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23078 | 5' | -52.6 | NC_005178.1 | + | 33585 | 0.66 | 0.783504 |
Target: 5'- gCGGCCGG-----GCC-UCGUCGUCGGu -3' miRNA: 3'- -GCCGGCCuuuagUGGuGGUAGUAGCU- -5' |
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23078 | 5' | -52.6 | NC_005178.1 | + | 33233 | 0.66 | 0.774148 |
Target: 5'- aGGCCGGGGAUggacagcggcgcaucCAuuuccagggcauacCCGCCGUCAcgcUCGGc -3' miRNA: 3'- gCCGGCCUUUA---------------GU--------------GGUGGUAGU---AGCU- -5' |
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23078 | 5' | -52.6 | NC_005178.1 | + | 35162 | 0.66 | 0.7731 |
Target: 5'- cCGGCUGGAGgacGUCACUgucgGCCAggccgcagUCGUgGAc -3' miRNA: 3'- -GCCGGCCUU---UAGUGG----UGGU--------AGUAgCU- -5' |
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23078 | 5' | -52.6 | NC_005178.1 | + | 4898 | 0.66 | 0.7731 |
Target: 5'- cCGGCCGGGAccaucccggaaAUgGCCgACCAgaUCGcCGAg -3' miRNA: 3'- -GCCGGCCUU-----------UAgUGG-UGGU--AGUaGCU- -5' |
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23078 | 5' | -52.6 | NC_005178.1 | + | 17408 | 0.66 | 0.7731 |
Target: 5'- uGGCCGGGcgcuauGCCGCCG-CAUaCGAg -3' miRNA: 3'- gCCGGCCUuuag--UGGUGGUaGUA-GCU- -5' |
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23078 | 5' | -52.6 | NC_005178.1 | + | 28552 | 0.66 | 0.762538 |
Target: 5'- gGGCCGuccgccgCGCCACCGggggCGUCGc -3' miRNA: 3'- gCCGGCcuuua--GUGGUGGUa---GUAGCu -5' |
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23078 | 5' | -52.6 | NC_005178.1 | + | 8610 | 0.66 | 0.751831 |
Target: 5'- -cGCCGGAAGUCuCCgGCCAagGUCa- -3' miRNA: 3'- gcCGGCCUUUAGuGG-UGGUagUAGcu -5' |
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23078 | 5' | -52.6 | NC_005178.1 | + | 20954 | 0.66 | 0.751831 |
Target: 5'- gCGGCaacguccaGGucuUCGCCggcGCCAUCGUCGu -3' miRNA: 3'- -GCCGg-------CCuuuAGUGG---UGGUAGUAGCu -5' |
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23078 | 5' | -52.6 | NC_005178.1 | + | 14240 | 0.66 | 0.740992 |
Target: 5'- gGGCCgcgucuGGcAAGUCGgCACCAUCGUUa- -3' miRNA: 3'- gCCGG------CC-UUUAGUgGUGGUAGUAGcu -5' |
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23078 | 5' | -52.6 | NC_005178.1 | + | 23458 | 0.66 | 0.740992 |
Target: 5'- gCGGCCGGugagUACgCACCAgaUCA-CGAa -3' miRNA: 3'- -GCCGGCCuuuaGUG-GUGGU--AGUaGCU- -5' |
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23078 | 5' | -52.6 | NC_005178.1 | + | 34596 | 0.66 | 0.740992 |
Target: 5'- gCGGCCGcGAgGAUCGCgGgCCAUC-UUGAg -3' miRNA: 3'- -GCCGGC-CU-UUAGUGgU-GGUAGuAGCU- -5' |
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23078 | 5' | -52.6 | NC_005178.1 | + | 16957 | 0.66 | 0.740992 |
Target: 5'- aGGCCGuccacUCGCCGCCGUUgaggCGAg -3' miRNA: 3'- gCCGGCcuuu-AGUGGUGGUAGua--GCU- -5' |
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23078 | 5' | -52.6 | NC_005178.1 | + | 21362 | 0.67 | 0.730034 |
Target: 5'- aGGCCGG---UCACCGCUcgCAgcaGGg -3' miRNA: 3'- gCCGGCCuuuAGUGGUGGuaGUag-CU- -5' |
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23078 | 5' | -52.6 | NC_005178.1 | + | 29844 | 0.67 | 0.730034 |
Target: 5'- uGGCUGGG--UCACCgACCGcgccucggCAUCGGa -3' miRNA: 3'- gCCGGCCUuuAGUGG-UGGUa-------GUAGCU- -5' |
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23078 | 5' | -52.6 | NC_005178.1 | + | 12883 | 0.67 | 0.730034 |
Target: 5'- aCGGUCGGGGAaCAgaacuuCCagGCUGUCAUCGAc -3' miRNA: 3'- -GCCGGCCUUUaGU------GG--UGGUAGUAGCU- -5' |
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23078 | 5' | -52.6 | NC_005178.1 | + | 34052 | 0.67 | 0.707812 |
Target: 5'- uGGCCGGGA--CGCCGCCcuugaugcaggcGUCAcguagggcaUCGAc -3' miRNA: 3'- gCCGGCCUUuaGUGGUGG------------UAGU---------AGCU- -5' |
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23078 | 5' | -52.6 | NC_005178.1 | + | 21579 | 0.67 | 0.696574 |
Target: 5'- aCGGCUGGAGuaccUCGCCcgcgauGCUGUUAUCGc -3' miRNA: 3'- -GCCGGCCUUu---AGUGG------UGGUAGUAGCu -5' |
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23078 | 5' | -52.6 | NC_005178.1 | + | 3643 | 0.67 | 0.696574 |
Target: 5'- aGGCCGccGAGcAUCGCCGCCAggcgCGGc -3' miRNA: 3'- gCCGGC--CUU-UAGUGGUGGUaguaGCU- -5' |
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23078 | 5' | -52.6 | NC_005178.1 | + | 8844 | 0.67 | 0.685268 |
Target: 5'- cCGGCCcacauGUCgGCCGCCAUCugGUCGGg -3' miRNA: 3'- -GCCGGccuu-UAG-UGGUGGUAG--UAGCU- -5' |
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23078 | 5' | -52.6 | NC_005178.1 | + | 17493 | 0.67 | 0.685268 |
Target: 5'- aGGCCGGAGccuUCG-CGCCAU--UCGAa -3' miRNA: 3'- gCCGGCCUUu--AGUgGUGGUAguAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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