Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23134 | 3' | -64.1 | NC_005178.1 | + | 10931 | 0.66 | 0.193489 |
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Target: 5'- cGCGCCgagUCGaucCGGCGcggcccgccuccuGCCCCCcgcgucACCACa -3' miRNA: 3'- uUGCGGa--AGC---GCCGC-------------CGGGGG------UGGUG- -5' |
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| 23134 | 3' | -64.1 | NC_005178.1 | + | 10885 | 0.66 | 0.193999 |
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Target: 5'- gGACGCCUUCGCGaaGGUCaaggcguaUCGCCAg -3' miRNA: 3'- -UUGCGGAAGCGCcgCCGGg-------GGUGGUg -5' |
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| 23134 | 3' | -64.1 | NC_005178.1 | + | 36166 | 0.66 | 0.193999 |
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Target: 5'- -uCGUCUUUGCGGCGggccauguucaaGCCCUCGCUuCg -3' miRNA: 3'- uuGCGGAAGCGCCGC------------CGGGGGUGGuG- -5' |
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| 23134 | 3' | -64.1 | NC_005178.1 | + | 3492 | 0.66 | 0.199168 |
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Target: 5'- uGACGCCUgcaucaaggGCGGC-GUCCCgGCCAUc -3' miRNA: 3'- -UUGCGGAag-------CGCCGcCGGGGgUGGUG- -5' |
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| 23134 | 3' | -64.1 | NC_005178.1 | + | 20136 | 0.66 | 0.204457 |
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Target: 5'- --gGCCUcguaUGCGGCGGCauagcgCCCgGCCAUg -3' miRNA: 3'- uugCGGAa---GCGCCGCCG------GGGgUGGUG- -5' |
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| 23134 | 3' | -64.1 | NC_005178.1 | + | 9055 | 0.66 | 0.209867 |
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Target: 5'- cGCGCCgcgcUCGCGGUcaccGGCCUCCAg--- -3' miRNA: 3'- uUGCGGa---AGCGCCG----CCGGGGGUggug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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