Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23134 | 3' | -64.1 | NC_005178.1 | + | 26162 | 0.7 | 0.092918 |
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Target: 5'- uAGCGCCUUuaucaucaucggCGCGGCGGUggCCGCUGCg -3' miRNA: 3'- -UUGCGGAA------------GCGCCGCCGggGGUGGUG- -5' |
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| 23134 | 3' | -64.1 | NC_005178.1 | + | 10398 | 0.7 | 0.092658 |
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Target: 5'- gAugGCCUUgcgaGCGGCGGCgauauuuUCCgGCCGCa -3' miRNA: 3'- -UugCGGAAg---CGCCGCCG-------GGGgUGGUG- -5' |
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| 23134 | 3' | -64.1 | NC_005178.1 | + | 22584 | 0.73 | 0.062578 |
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Target: 5'- aGGCGCUUgCGgaUGGCGGCCUCgACCACg -3' miRNA: 3'- -UUGCGGAaGC--GCCGCCGGGGgUGGUG- -5' |
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| 23134 | 3' | -64.1 | NC_005178.1 | + | 11362 | 0.73 | 0.059113 |
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Target: 5'- cAGCGCCacgccCGCaGCGGCCaCCGCCGCg -3' miRNA: 3'- -UUGCGGaa---GCGcCGCCGGgGGUGGUG- -5' |
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| 23134 | 3' | -64.1 | NC_005178.1 | + | 8994 | 0.76 | 0.031411 |
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Target: 5'- cGACGCCcccgguggCGCGGCggacGGCCCCCGCC-Ca -3' miRNA: 3'- -UUGCGGaa------GCGCCG----CCGGGGGUGGuG- -5' |
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| 23134 | 3' | -64.1 | NC_005178.1 | + | 21372 | 1.05 | 0.000139 |
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Target: 5'- cAACGCCUUCGCGGCGGCCCCCACCACc -3' miRNA: 3'- -UUGCGGAAGCGCCGCCGGGGGUGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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