Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23191 | 5' | -52.2 | NC_005259.1 | + | 8767 | 0.66 | 0.887737 |
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Target: 5'- uGAGCUCGCuggcacGCUUGCGuGCGGguGCa- -3' miRNA: 3'- -CUCGAGCG------UGGACGCuUGUUguUGcu -5' |
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| 23191 | 5' | -52.2 | NC_005259.1 | + | 66379 | 0.66 | 0.887737 |
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Target: 5'- uGGCacuacCGCACCUcGcCGAACAAgGGCGGu -3' miRNA: 3'- cUCGa----GCGUGGA-C-GCUUGUUgUUGCU- -5' |
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| 23191 | 5' | -52.2 | NC_005259.1 | + | 16466 | 0.66 | 0.894324 |
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Target: 5'- uGGGC-CGCGCCccaagauuggaugUGCGAGCccGACAugGu -3' miRNA: 3'- -CUCGaGCGUGG-------------ACGCUUG--UUGUugCu -5' |
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| 23191 | 5' | -52.2 | NC_005259.1 | + | 65431 | 0.66 | 0.895042 |
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Target: 5'- cGAGCUCGcCGCCUacaaGGACAuCGACa- -3' miRNA: 3'- -CUCGAGC-GUGGAcg--CUUGUuGUUGcu -5' |
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| 23191 | 5' | -52.2 | NC_005259.1 | + | 24770 | 0.66 | 0.895042 |
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Target: 5'- cGAGCUCGCccgccgcgccGCCcGCGcGCGAgaaguCGGCGAc -3' miRNA: 3'- -CUCGAGCG----------UGGaCGCuUGUU-----GUUGCU- -5' |
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| 23191 | 5' | -52.2 | NC_005259.1 | + | 22423 | 0.66 | 0.902074 |
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Target: 5'- cGGCgucgacCGCGCCgcccgagGCGAGCAGCGcgucguugGCGGu -3' miRNA: 3'- cUCGa-----GCGUGGa------CGCUUGUUGU--------UGCU- -5' |
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| 23191 | 5' | -52.2 | NC_005259.1 | + | 2041 | 0.66 | 0.902074 |
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Target: 5'- cGAGCUguuucuugCGCGCCUcGCGGugAAUcGCGu -3' miRNA: 3'- -CUCGA--------GCGUGGA-CGCUugUUGuUGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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